| Objective：To detection the level of serum25-dihydroxyvitamin D3in childhood acute leukemia and to explore its clinical significance，as thejudgment condition,evaluation of curative effect, guide clinical treatment andevaluating prognosis provide certain theoretical basis.Methods:1Cases and groups：This research was randomly selected60cases newlydiagnosed and untreated with childood acute leukemia to be hospitalized in theblood group of department of pediatrics in the second hospital of Hebeimedical university from August2012to August2013,those treated as theuntreated group of the experiment. Among them there were30children withacute lymphoblastic leukemia(ALL), after a month from first chemotherapy,27cases had achieved complete remission，which was treated as remission onemonth group, then after six months with treatment observation, all the27cases reached continued complete remission and treated them as remission sixmonths group.And the other30children with acute myeloid leukemia(AML)after a month from first chemotherapy,24cases had achieved completeremission，which was treated as remission one month group, then after sixmonths with treatment observation,22cases reached continued completeremission and treated them as remission six months group.Meanwhile, as the1:1matching principle, choosed the healthy children with same factor likegender, age, body height and weight as control group. All the children in theresearch should have not any disease of heart, brain, kidney and endocrine.They also had not the sexual development.2Sample collection, handling and the data measurement: All of objectswere extracted2ml fasting venous blood in the morning of6:00to8:00. Allvenous blood were placed in the promoting coagulation tubes, and then put them in the ordinary centrifugal machine, with a speed of2500r/min after10min, serum samples were collected. Divided the samples equally and keptthem in the fridge at-80℃. Measured the level of serum25-dihydroxy vitaminD3.3statistics analysis: SPSS13.0was used, and the data was described asmean±SD. Analysis methods include analysis of variance. P value of less than0.05was considered as statistical significance.Results:Serum25-dihydroxyvitamin D3levels:t the level was (30.95±6.32)nmol/L in untreated group(AL), the level was(51.32±14.62) nmol/L inremission one month group(AL), the level was (40.13±9.92) nmol/L inremission six month group(AL), he level was (30.51±13.81) nmol/L inuntreated group(ALL), the level was(61.12±18.93) nmol/L in remissionone month group(ALL), the level was (44.43±12.60) nmol/L in remission sixmonth group(ALL), the level was (31.44±7.13) nmol/L in untreatedgroup(AML), the level was(41.24±10.29) nmol/L in remission one monthgroup(AML), the level was (36.73±9.73) nmol/L in remission six monthgroup(AML),the level was (58.71±8.97) nmol/L in normal control group. Thedifferences betwteen untreated group(AL) and normal control group hadstatistical significance (P <0.05); and the level had not statistical significancebetween remission one month group(AL) and normal control group (P <0.05);and the level had statistical significance between remission six monthgroup(AL) and normal control group (P <0.05);The differences betwteenuntreated group(ALL) and normal control group had statistical significance(P <0.05); and the level had not statistical significance between remissionone month group(ALL) and normal control group (P>0.05); and the levelhad statistical significance between remission six month group(ALL) andnormal control group (P <0.05); the differences betwteen untreat-edgroup(AML) and normal control group had statistical significance (P <0.05);the level had not statistical significance betwteen untreated group(AML)group and remission one month group(AML)(P <0.05); the level had not statistical significance betwteen untreated group(AML) group and remissionsix month group(AML)(P>0.05); the level had not statistical significancebetwteen remission one month group(AML) and remission six monthgroup(AML)(P>0.05)；the level had not statistical significance betwteenuntreated group(ALL)and untreated group(AML)(P>0.05); the level hadstatistical significance betwteen remission one month group(ALL) andremission one month group(AML)(P <0.05); the level had not statisticalsignificance betwteen remission six month group(ALL) and remission sixmonth group(AML)(P>0.05).Conclusion:1The levels of serum25-dihydroxy vitamin D3in untreated group wasdistinctly lower than the control group, so they could become a kind ofdetection index of acute leukemia.2After complete remission in childhood acute leukemia, the levels ofserum25-dihydroxy vitamin D3gradually rose,but with the development ofthe treatment the levels of serum25-dihydroxy vitamin D3gradually fluctuate.We suggested that dynamic detection the levels of serum25-dihydroxyvitamin D3in childhood acute leukemia in different stages of treatment werecontributived to judge development of disease and assess treatment effect. |
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