Study On Ethyl Acetate Parts Of Hemostatic And Anti-inflammatory Effect Of Sedum Aizoon L.Activity Screening

ObjiectiveThe objiective of this project was to study the Monomer separation of the ethyl acetate part in Sedum aizoon L. On this fundation,we filtered out the hemostatic and anti-inflammatory active ingredients.This experiment was to provide theoretical basis for the clinical application of the effect of hemostatic and anti-inflammatory in Sedum aizoon L.Methods1.The ethyl acetate part were separated using chromatographic technique of silica gel,sephadex-20,HPLC and their structures were determined by the physical and chemical property and spectra of H-NMR,C-NMR and MS.2.The screening of hemostatic efficacy:capillary method of cultivating Sedum aizoon L.parts separation of ethyl acetate monomer effect on coagulation time (CT) of mice;Instrumental method of cultivating the separation of ethyl acetate monomer effect on prothrombin time (PT),activated partial thromboplastin time (APTT) and thrombin time (TT) of mice.3.The screening of anti-inflammatory efficacy:MTT assay for detection of cultivating Sedum aizoon L.parts separation of ethyl acetate monomer effect on RAW264.7mouse mononuclear phagocyte and the LPS stimulation the cell;Nitrate reduction test of cultivating Sedum aizoon L.parts separation of ethyl acetate monomer effect on LPS stimulation the cell release NO;ELISA method of cultivating Sedum aizoon L.parts separation of ethyl acetate monomer effect on LPS stimulation the cell release TNF-a, IL-iB and IL-6.Results1.The Sedum aizoon L.parts separation of ethyl acetate monomer were gallic acid,p-hydroxybenzoic acid,protocatechuic acid,vanillic acid,caffeic acid,5,7-two-color ketone,quercetin,kaempferol and luteolin.2.Yangxin grass ethyl acetate parts of the separation of phenolic acid compounds as gallic acid, vanillic acid and mignonette hemostatic effect, and gallic acid (including300g/mL), vanillic acid (including100g/mL) and mignonette (100, including30g/mL) optimal hemostatic effect. 3.Cultivating Sedum aizoon L.parts of ethyl acetate and separation of protocatechuic acid,vanillic acid,caffeic acid,quercetin and luteolin had anti-inflammatory efficacy.Conclusionl.The gallic acid,p-hydroxybenzoic acid,protocatechuic acid,vanillic acid and caffeic acid had hemostatic efficacy many activate many kinds of cruor factor in endogenous coagulation and extrinsic coagulation,and shorten CT、APTT、TT.2.The protocatechuic acid,vanillic acid,caffeic acid,quercetin and luteolin had anti-inflammatory efficacy may inhibite the generation of NO、TNF-a、IL-1B、IL-6pro-inflammatory cytokines.