| Objective: Hepatic venous obstruction is not uncommon with thedevelopment of living donor liver transplantation and extended liver resectionwith transaction of the major hepatic vein.This lead to focal congestion in thegraft or remnant liver. The purpose of this study was to establish partialhepatectomy with left lateral lobe congestion(congestion and non-congestionaccounted for the same volume),and then investigate the effect of livercongestion on liver regeneration and liver function after partial hepatectomyin rats.Method: A sample of40rats were divided into two groups, Group A:after the middle of liver lobe,blocking the left lateral lobe of the leaf veinscause congestion; Group B: Seventy percent Partial hepatectomy. At differenttime points (1day,2days,3days,4days) collecting blood and liver samplesto do the following detection:1. Portal venous pressure;2.weighing remnantliver weight, body weight and measuring the degree of liver regeneration;3.AST(aspartate aminotransferase) and ALT (alanine enzyme) in serum;4.remnant liver tissues were fixed, embedded, sectioned, stained, observingthe pathological changes and liver cell mitotic index in liver tissue withmicroscope;5.The method of imunohistochemistry was used to detect theexpression of proliferating cell nuclear antigen (PCNA);6.nzyme-linkedimmunosorbent assay (ELISA) detection of serum TNF-α (TNF-α), IL-6(interleukin-6) levels;7. The results of group A and group B were analyzedusing SPSS13.0statistical software,observe comparison of various indicatorsof group differences in liver regeneration(independent samples t test). Results: Portal venous pressure: the experimental group portal pressureare higher(p<0.05).2. Postoperative visual observation of the twogroups:residual normal liver tissue are noticeable increase in size overtime,and color of the experimental group congestion of liver tissue aregradually from dark red to yellow necrotic tissue.3. The degree of liverregeneration: the experimental group after the first day and the fifth day thereare two peaks,in the control group increased gradually over time, after thefirst day of the degree of liver regeneration in the experimental group than thecontrol group (P <0.05), postoperative The next day, the third day, fifth dayfive significant difference (P>0.05).4. Hepatocyte mitotic index: thecongestion area of experimental group with time lapse liver tissue developdegeneration and necrosis, loss of normal liver tissue morphology. In thecontrol group had a peak liver mitotic index after the second day, the third dayof a peak in the experimental group.5. Liver function markers: Both of thegroup of the AST, ALT reached a peak in the first postoperative day, and thengradually decreased in the experimental group at each time point were higherthan in the control group (P <0.05).6. PCNA index: peak in the experimentalgroup on the third day after surgery, and the peak is lower than the controlgroup.7. TNF-α, IL-6levels: the control group peaked on the firstpostoperative day, and gradually reduced, after the third day, the consent ofexperimental group than the control group (P <0.05).Conclusions1Partial liver resection with left lateral lobe vein occlusion in rats, thismodel is stable and repeatable rate, can be used for experimental studies.2Independent hepatic congestion will increase liver damage, to acertain extent,liver damage inhibits normal tissue regeneration in the early. 3The mechanism：①Independent hepatic vein occlusion in rats,probably because of increased portal pressure leading to a "portal venousreflux" phenomenon, but this mechanism can not be established a effectivereturn channel in the early time, so congestion tissue become deformation,necrosis, and can not maintain normal liver tissue structure and function, andaffect the regeneration of the residue liver.②The factor of regulating liverregeneration,such as TNF-α, IL-6, express abnormally;③The increasedendotoxin and inflammatory mediators cause the remnant liver injury. |
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