The Expression Of DNMT1and SATB1and The Significance Of Them In The Bladder Urothelial Carcinoma

Background and purpose:This study regard the bladder urothelial carcinoma patients with carcinoma tissue and normal bladder tissue from healthy person as the research object, used RT-PCR and immunohistochemical method, in order to test the expression of DNMT1and SATB1mRNA,and the protein expression level of DNMT1and SATB1in bladder urothelial carcinoma tissue and in the healthy people. And then have relativity comparison.Materials and methods:Collect the specimen from April2012to February2013in the urlology surgery in the Xinxiang Medical Collage First Affiliated Hospital. The50cases of carcinoma tissue were from the patients who had been postoperative pathology diagnosed with bladder urothelial carcinoma and the patients didn’t have chemotherapy and radiation before surgery, and take another15cases of normal bladder tissue from healthy person as control (the bladder microscopical examination were obtained by the agreement of the patients). Each person’s tissue specimen was divided into two parts, one part had be taken into liquid nitrogen storage in order to store for the experiment of RT-PCR to detect and observe DNMT1, SATB1mRNA expression of the bladder urothelial carcinoma tissue and normal bladder mucous membrane tissues. The other part of the specimen is fixed with paraformaldehyde, and then routine dehydration, paraffin embedding,3um serial section, and drying by baking, dewaxing, hydration, and had streptomycin resistance of biotin-peroxidase (streptavidin peroxidase, SP) immunohistochemical detection, to detect the DNMT1, SATB1protein expression level in the bladder urothelial carcinoma tissue and normal bladder mucous membrane tissues; and to discuss the relationship between the clinical pathological grading and staging and the expression of DNMT1and SATB1; the relationship between protein expression in bladder urothelial carcinoma of DNMT1and SATB1. Each section were randomly selected five nonoverlapping high power lens, observed and counted positive staining cell number respectively by two observers who don’t know the groups of the sections. Carry on the analysis by using AB value method (half quantitative method).Results:RT-PCR results show that DNMT1and SATB1genes in bladder urothelial carcinoma tissues were compared to the normal bladder tissue, and the expression quantity increased obviously, have the remarkable statistical differences (P<0.05).The results of the SP method of the immunohistochemistry showed that the positive expression rate of SATB1and DNMT1in bladder urothelial carcinoma tissue was for66.00%and64.00%respectively, and the corresponding control of normal bladder tissue were compared, the expression of SATB1and DNMT1increased significantly, and has significant statistical difference (P<0.05).The relationship between the expression of SATB1and DNMT1in bladder urothelial carcinoma and the clinical pathological grading and staging were as follow: In the patients who have pathologic stage high level, infiltrating or metastasis of tumor, the positive rate of the expression of SATB1and DNMT1,was obviously higher than in the patients that of the pathological grade level was low, the tumor was superficial, or no tumor metastasis (P<0.05).The positive expression rate for SATB1in bladder urothelial carcinoma tissue was significant correlated (r=0.341, P<0.05) with DNMT1in bladder urothelial carcinoma tissue.Conclusions:1. DNMT1and SATB1genes had over expression in bladder urothelial carcinoma tissues2. DNMT1, SATB1protein had high expression in the bladder urothelial carcinoma tissue, and closely related with pathologic stage, invasive and metastatic.,3. SATB1and DNMT1protein were positively related in the bladder urothelial carcinoma tissues.