| Objective:Study and disscuss the difference and meaning in theexpression of STIM1which is the key protein in calcium release-activatedcalcium(CRAC) in young and adult rats with posttraumatic epilepsy.Methods: SD male adult and young rats were taken each94only,and randomized into adult and young Sham-operated group(whichwere injected by saline into the cortex each42only)and modelgroup(which were injected by FeCl3into the cortex each52only).Thebehavioral changes of the model group were carefully observed. After6,24,72h and7,14,21,28d of the injection,the rats were sacrificed and gotthe cortex tissue which was around the injection site.6rats were randomselected at each time point.Real-time fluorescence quantitativePCR(RT-PCR),western blot and immunohistochemistry were used tomeasure the expression of STIM1of the cortex.Results:1.Epileptic seizure was found only in the model group ofadult and young rats. In the models with epilepsy induced by FeCl3,theachievement ratio of young group was91.3%, but adult group only84%(the definition of the successful model was that the Racine score was3 and above).2.Compared with the model group of adult rats,the modelgroup of young rats had singnificantly more epileptic seizures(P<0.05),also had significantly higher severity and longer duration ofepileptic seizure(P<0.05).3.The expression of STIM1in the model grouphad significantly more than the Sham-operated group(P<0.05) in each timegroup,and72h group was the highest one(P<0.05).Compared with themodel group of adult rats,the expression of STIM1in the model group ofyoung rats increased significantly in each time group(P<0.05).Conclusion: Young rats had posttraumatic epileptic seizures easierand higher severity than adult rats. The expression of STIM1in young ratswas higher than in adult rats,suggesting that higher expression of STIM1and calcium release-activated calcium(CRAC) activated minght be one ofthe pathogenesis that young rats had easier posttraumatic epileptic seizures. |
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