| Objectives: 1.To observe the kinesis expression of nestin, nuclear factor-kB (NF-kB) and glial fibrillary acidic protein (GFAP) in the CA3 area of hippocampus of the experimental posttraumatic epilepsy (PTE) in rats. To learn the oxidative stress response after PTE and the dynamic changes of glial cells and neural stem cells.2.To observe the dynamic changes of CD133 which is neural stem cell-specific indicator. Relevant indicators of glial cells were detected by double label immunohistochemistry to observe the pathological changes. To clarify the possible ways of glial cell proliferation after PTE.3.To intervene these above with the new kind of antioxidants edaravone. And comparied the the expression of CD133, NF-kB and GFAP to explore possible prevention and cure mechanisms of PTE.Methods: 1.29 healthy adult male Sprague-Dawley rats were divided into the saline control group and the epilepsy model group using random number table. PTE models were made by healthy adult male Sprague-Dawley rats with FeCl2(0.1mol/L,10uL) injection into the motor cortex. Behavior was observed and EEG were recorded, brains were removed at different time points. The expression of nestin, NF-kB and GFAP in the CA3 area of hippocampus were detected immunohistochemically with the professional image analysis software to observe the pathological changes.2.Further more, the expression of CD133 were detected immunohistochemically. CD133/NF-kB and GFAP/NF-kB were detected by double label immunohistochemistry to observe the pathological changes. To observe the the trends of the expression of glial cells and neural stem cells.3. 36 healthy adult male Sprague-Dawley rats were divided into the normal group, the control group and the treatment group using random number table. Intraperitoneal injection of edaravone(3mg/kg) was enforced to PTE models of the treatment group. Behavior was observed and EEG were recorded in each group, brains were removed at different time points. The expression of CD133, NF-kB and GFAP in the CA3 area of hippocampus were detected immunohistochemically to observe the pathological changes.Results: 1. 17 PTE models had been made successfully, 2 of which died of status epilepticus due to suffocation after 1-3 hours. The success rate of modeling is 88.24%. The expression of nestin in the CA3 area of hippocampus of PTE model group gradually increased at one day after injection and increased significantly 7 days later,and decreased after 14 days. The expression of NF-kB sharply increased one day after injection, decreased in 7 days later and significantly decreased after 14 days. The expression of GFAP increased obviously after 7 days and this high expression level was maintained till 14 days (P<0.05).2.The expression of CD133 of PTE model group increased at one day after injection and increased significantly 7 days later , then it decreased after 14 days. The expression of CD133/NF-kB double label couble both be detected one day after injection, then kept on increasing in 7 days. But there was no nuclear and cytoplasm co-staining cell. Positive cells significantly decreased after 14 days. Co-expression of GFAP/NF-kB double label gradually increased one day later and this high expression level was maintained till 14 days.3.Compared to normal group and control group, the expression of CD133,NF-kB and GFAP of treatment group were weaker at each time piont. (P<0.05)Conclusions: 1.The proliferation and differentiation of neural precursor cells were induced after PTE. Moreover, the self-proliferation and hyperplasia of glial cells may play an important role in the epileptogenesis of PTE.2.In PTE models induced by cortical injection of ferrous chloride, NSCs differertiation and glial cells self-proliferation produce a large number of glial cells in the hippocampal. 3.Some reactions after PTE could lead structure disorder. Edaravone can effectively alleviate the patho-reconstitution process, playing an important role in the prevention and cure in late PTE. |
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