Study On Xinjiang Tuinip Total Flavonoids Of Relieving Cough And Asthma, Expectorant Effect

Objective:1.To study the optimal extraction and purification condition of totalflavonoids from Brassica rapa L. and determine the concentration.2. To study theantitussive, antiasthmatic, expectorant and anti-inflammatory actions of the totalflavonoids from Brassica rapa L. preliminary.3.To study the optimal extraction process ofglucosinolates from Brassica rapa L. and determine the concent. Methods:1.On the baseof single factors experiments, define the optimal extraction process of total flavonoidsfrom Brassica rapa L. by L₉（3⁴） orthogonal experimental design and determine thecontent by UV Spectrophotometry. Taking adsorption capacity,desorption capacity,thedesorption ratio as index, evaluate the purification ability of these resins by the staticabsorption, dynamics absorption and desorption experiments.2.To evaluate the antitussive,expectorant, anti-inflammatory, antiasthmatic actions and toxicity of the total flavonoidsby concentrated ammonia water spraying experiment, sulfur dioxide stimulating method,phenolsulfonphthalein excreting method, inserting capillary glass tube to trachea, methodof inducing auris swell by dimethylbenzene and inducing foot swell by egg white,inducing asthma attacks by histamine and acetylcholine, morphological changes of thelungs and acute toxicity test.3. On the base of single factors experiments, define theoptimal extraction process of glucosinolates from Brassica rapa L. by L₉（3⁴） orthogonalexperimental design and determine the content by Sulfate ion precipitation method.Results:1. By means of orthogonal experimental and variance analysis obtain theoptimum condition: the ethanol concentration was75%, extraction time was2.5hour,extraction temperature was80℃, solid to liquid ratio was1：60. The HPD^-100resin hadoptmial parameters of absorption and elution.The optmium technology was as follows: therate of diameter to height is1:10, sampling flow rate2ml/min, the concentration of thesample （raw medicine） was0.2400mg·mL^-1, eluting with95%ethanol（1VB）, repeatedadsorption for1time.2.The total flavonoids from Brassica rapa L. can prolong incubation period of cough reaction and reduce cough score in mice and increase theexcretion of phenolsulfonphthalein, inhitite the mouse auricle tumefaction degree inducedby dimethylbenzene and the the paw edema volume induced by egg white in mice. It alsoeffective to prolong incubation period of asthma in SD ratsim and prove morphologicalchanges of the lungs in the asthmatic model. The Lethal Dose50test （LD50）was not sure,and the maximum tolerance dose（MTD） in mice was567.27g/kg.3. L₉（3⁴） orthogonal testand analysis of variance obtained from the Brassica rapa L. extract glucosinolatesoptimum conditions were: extraction times of1, the extraction time was120min, thesolid-liquid ratio of1:10. Conclusion: The results shows that the method to extract andpurify the total flavonoids and the optimal extraction process of the glucosinolates areeasy and reliable to operate and have the extensive applicability and practical significanceto study Brassica rapa L.. The total flavonoids of Brassica rapa L. had obvious effects ofantitussive, antiasthmatic, expectorant and anti-inflammatory without acute toxicity.