Experimental Study Of The CV Abstracted From Paris Polyphylla Which Inhibited The Lung Adenocarcinoma Cell Line XWLC-05from Yunnan Xuanwei

[Objective]:This study was designed to primarily investigate the inhibitory effects and the mechanisms of CV abstracted from Paris polyphylla Smith of Yunnan on lung cancer cell line XWLC-05and to eluminate its potential mechanism in apoptosis.[Methods]:1. MTT (Methy thiazolyl tetrazolium) assay was used to evaluate the inhibitory effects of XWLC-05and KMB-17cells induced by CV and CDDP in different time and different concentration, and each one is divided into four groups of negative control group、solvent control group and experimental group (with the CV)、positive control group (with the CDDP),and then to calculate the half inhibiting concentration(IC50).2. XWLC-05cells were induced by CV and CDDP in24hours, Key GEN living cells/apoptotic cells/necrotic cells identification kit(AO/EB method)by fluorescence microscopy was used to observate apoptosis of the cells.3. XWLC-05cells were induced by CV and CDDP in24hours and then put in fixed liquid, electron microscope was used to scan the cells.4. DNA ladder of XWLC-05cells were induced by CV and CDDP in24hours was tested by GENMED DNA testing kit. 5. All data were analyzed by SPSS17.0software and statistics were expressed as mean±standard deviation (x±s), single factor analysis of variance (ONE of WAY ANOVA) and t test were used to get the statistical significance.[Results]:1. CV and CDDP could inhibit the proliferation of XWLC-05and KMB-17cells, the values of IC50for each group are exhibited as following table:There is no statistic significancance between cisplatin and pairwise group (P>0.05), but abvious difference of IC50was found in CV cells betwen XWLC-05and KMB-17treatment (P=0.0217; p<0.05).2. The apoptosis XWLC-05cells induced by CV and CDDP at24hours were found by fluorescence microscope with AO/EB method.3. Apoptotic bodies of XWLC-05induced by CV and CDDP at24hours were deteced by electron microscopy images.4. DNA ladders were found in XWLC-05and KMB-17cells treated with GENMED at24hours.[Conclusion]:1. CV could inhibit the proliferation of the XWLC-05cells in vitro, its proliferation is on time and dose dependment.2. CV could inhibit the proliferation of the XWLC-05cells in vitro,apoptosis in the early formation of crescent-shaped cells, and also the formation of apoptosis in late cell cytoplasm bud-like protuberances.3. CV could inhibit the proliferation of the XWLC-05cells in vitro, apoptotic bodies of cells can form.4.CV can induce XWLC-05cells apoptosis and form fracture bands, but to KMB-17it can not form the fracture bands within a certain concentration.