Detection Of Volatile Organic Compounds Emitted From The Lung Tissues Infected By Some Pathogens

Objective:Through the detection of the characteristic volatile organic compounds(VOCs) emitted from the lung tissues infected by some pathogenic bacteria.to provide the experimental basis for the application of respiratory gas detection in the early diagnosis of pulmonary infectious diseases.Methods:To establish the model of bacteria infecting lung organization and the model of bacteria infecting animal.then detect the characteristic VOCs emitted from the models infected by Escherichia coli.Staphylococcus aureus and Pseudomonas aeruginosa,using solid-phase microextraction and gas chromatography mass spectroscopy (SPME/GC-MS). Results:C12H26,C14H22and hexadecanol(C16H34O) can be detected in the model of Escherichia coli infecting lung organization,C12H26,C14H22and C14H22O can be detected in the model of Staphylococcus aureus infecting lung organization,while C12H26,C14H22and C18H30O can be detected in the model of Pseudomonas aeruginosa infecting lung organization.During the model of bacteria infecting animal,the biomarker of Escherichia coli can find to be C16H34O, with that of Staphylococcus aureus C14H22O and that of Pseudomonas aeruginosa C18H30O.Conclusion:By comparing the results of two different kinds of bacteria infecting models,we can find that caracteristic VOCs can be emitted from the lung tissues infected by Escherichia coli,Staphylococcus aureus and Pseudomonas aeruginosa. C16H34O can be the biomarker of Escherichia coli, while the biomarker of Staphylococcus aureus can be C14H22O and that of Pseudomonas aeruginosa C18H30O.