Essential Oil From Lemon Via Olfactory Pathway Prevents DXM-induced Weight-gain In Mice

BackgroundModern civilization is faced with an epidemic of overweight and obesity whichnow affects nearly one-third of the world’s population, and it is continuing to rise.Obesity is a major health problem world-wide and it pose major risks for seriousdiet-related chronic diseases, including type-2diabetes, cardiovascular disease,hypertension, stroke, musculoskeletal disorders such as osteoarthritis, and certain formsof cancer. Nowadays there are many types of interventions for obesity, such as drugs,surgery, and other sports, but the effect is not ideal; So how can safely and effectivelyreduce weight has become a hot issue of global concern. Olfactory system providesdirectly linked to the brain and the external environment, and with many of the brain incharge of feeding areas of the brain are closely linked. This experiment throughlong-term extensive use of dexamethasone cause obesity in mice, using smell affectappetite, explore the olfactory pathway could be potentially effective way to reduceweight, so as to achieve the effect that reduce weight.Object：Using the link between olfactory pathway and the feeding behavior, explore lemonessential oil’s effect on body weight in mice; evaluate its role in the treatment ofdexamethasone-induced obesity. Provide theoretical basis for the olfactory pathway treatment of obesity, open up new areas of obesity treatment, support and provide basictheory for aromatherapy.Part One: GC/MS Analysis of Lemon Essential OilMethodsThe lemon essential oil dissolved in hexane, match into2%solution, each sample1ul, flows through the DB-5capillary column, using GC/MS technology and compareswith NIST standard spectral library determine the chemical composition in the lemonessential oil, according to peak area normalization method percentage of componentsare obtained.ResultsThe GC/MS analysis of the essential oil of lemon led to the identification andquantification of approximately12main compounds, which accounted for98.85%ofthe total components present. m-Mentha-6,8-diene,(R)-(+)-(77.04%) was the principalcomponent followed by-Terpinen (14.90%).Part two: Body Weight of Dexamethasone-induced Mice Observed after SuckLemon Essential OilMethodsMice were divided into DXM clean air group and DXM lemon essential oil groupand blank group. Given DXM clean air group and DXM lemon essential oil groupdexamethasone injection(1ml:5mg), according to body weight in mice abdominalcavity injection, once every three days, at a time of0.02ml/g. Every three days torecord body weight of mice. Weight more than the original weight20%as buildingsuccess. To2g of lemon essential oil dissolved in1000ml hot water, after put DXMlemon essential oil group in airtight canister box1h, for3w. Observe three groups weight change, and use statistical software to analyze data is statistically significant ornot. Through the weight data to explore whether lemon essential oil can impact onweight via the olfactory pathway.ResultsThrough the weight data show: DXM lemon essential oil group relative to DXMclean air group reduce weight significantly, after statistical software analysis, P <0.05proved to be statistically significant. Lemon essential oil can be via the olfactorypathway has had an effect on body weight in mice.Part three: Hypothalamus Nuclei and Neurotransmitter Changes Observed afterSuck of Lemon Essential OilMethodsWeight data and the line chart shows that the weight of DXM lemon essential oilgroup mice were significantly decrease (P <0.05). After to see weight loss thehypothalamus and neurotransmitter changes, by the method of pathology, the lemonessential oil absorption in mice after smelling the hypothalamus area as well as thechanges of expression level, body weight of mice.ResultsBe testing, did not display the resultsConclusion1. GC/MS analysis found that12kinds of chemical composition in lemon essential oil,accounting for98.85%of the total essential oil content, m-Mentha-6,8-diene,(R)-(+)-is the highest content of oil and accounts for77.04%of the proportion of theingredients. 2. DXM obese mice after sucking lemon essential oil by olfactory pathway can reducebody weight in mice. There is clearly established effect for feeding center ofhypothalamus in mice.3. Lemon essential oil contain some compounds can lead to weight reduction and thesecompounds could impact weight by olfactory pathway.4. After suck lemon essential oil whether effect the hypothalamus in the feeding centerof mice or not and influence related neurotransmitter changes that cause weight loss isstudying.