The Expression And Role Of CD133, β-catenin And HTERT In Human Colorectal Cancer

Objective To explore the relationship between the expression of CD133, P-catenin, hTERT and pathoclinical parameter in human colorectal cancer (CRC).Methods The transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry were adopted to detect the expression of CD133,β-catenin and hTERT in30panels of samples including cancerous tissues and tissues of cutting side,9normal colorectal tissues and30skin tissues without any tumor. All the results were statistically analyzed.Results1. Both of the mRNA transcriptional level and protein expression of CD133, P-catenin and hTERT expression in tumors were higher than that in tissues of cutting side, normal colorectal tissues and30skin tissues without any tumor.2. The mRNA transcriptional level of CD133and β-catenin in tumor tissues of lymph-node positive group was significantly higher than those in lymph node negative group (P<0.05). The mRNA transcriptional level of CD133and β-catenin in tumor tissues of distant metastasis positive group was significantly higher than that in distant metastasis negative group(P<0.05). The mRNA transcriptional level of CD133and β-catenin in tumor tissues of stage Ⅲ+Ⅳ group was significantly higher than that in stage Ⅰ+Ⅱ (P<0.05). While there was no significant difference in gender, age, degree of tumor differentiation and invasion depth. The mRNA transcriptional level of hTERT in tumors of poorly and undifferentiationed group was significantly higher than those in highly and moderately differentiated group(P<0.05). The mRNA transcriptional level of hTERT in tumors of distant metastasis positive group was significantly higher than that in distant metastasis negative groups(P<0.05). There was no significant difference in gender, age, lymphatic metastasis, invasion depth and TNM staging.3. The protein expression level of CD133in tumor tissues of distant metastasis positive group was significantly higher than that in distant metastasis negative group(P<0.05). The protein expression level of CD133in tumor tissues of invasion depth T3+T4staging was significantly higher than that in T1+T2 staging (P<0.05). While there was no significant difference in gender, age, lymphatic metastasis, degree of tumor differentiation and TNM staging. The protein expression level of β-catenin in tumor tissues of lymph node positive group was significantly higher than those in lymph node negative group (P<0.05) The protein expression level of β-catenin in tumor tissues of distant metastasis positive group was significantly higher than that in distant metastasis negative group(P<0.05). The protein expression level of β-catenin in tumor tissues of stage Ⅲ+Ⅳ group was significantly higher than that in stage Ⅰ+Ⅱ(P<0.05). While there was no significant difference in gender, age, degree of tumor differentiation and depth of tumor invasion. The protein expression level of hTERT in tumors of poorly and undifferentiationed group was significantly higher than those in highly and moderately differentiated group(P<0.05). The protein expression level of hTERT in tumors of distant metastasis positive group was significantly higher than that in distant metastasis negative group(P<0.05). The protein expression level of hTERT in tumor tissues of depth of tumor invasion T3+T4staging group was significantly higher than that in T1+T2staging(P<0.05). There was no significant difference in gender, age, lymphatic metastasis and TNM staging.4. There was positive correlation between the mRNA transcription and protein expression of CD133, β-catenin and hTERT in CRC.Conclusion1. The expression of CD133,β-catenin and hTERT in CRC had siginificantly difference in different tumor invasion depth, dgree of differentiation, lymphatic metastasis, TNM staging and distant metastasis, indicating that increase of the three indexes was related to the tumor invasion and metastasis, and also could be used to evaluate to prognosis of CRC.2. The increase expression of hTERT in CRC could maintain the stability of telomere by activating telomerase in tumor cells in order to gain immortalization for tumors. The whole process would promote the malignant transformation.3. The increase of CD133, β-catenin and hTERT expression in CRC indicated cancer stem cell might activate Wnt signaling pathway to activate the telomerase in tumor cells in order to gain immortalization and promote the malignant transformation of tumors which would break the balance between apoptosis and proliferation and lead to the invasion and metastasis of CRC.4. There was positive correlation between the mRNA transcription and protein expression of CD133, β-catenin and hTERT in CRC. It suggested that Wint signaling pathway played a part in mRNA transcription.