The Expression And Significance Of HPV-16 E7 And HTERT In Cervical Carcinoma

ObjectiveTo detect the expression of Human papilloma virus 16(HPV-16) E7 and human telomerase reverse transcriptase (hTERT) in chronic cervicitis(CC), cervical intraepithelial neoplasia(CIN) and cervical squamous cell carcinoma(CSCC), analyze their association with the pathological features. And to further explore the role of HPV-16 E7 and hTERT in the development of the cervical carcinoma, with their correlation.MethodsHPV-16 E7 and hTERT expression was detected by using SP immunohistochemical assay on paraffin-embedded sections from 15 cases of CC,17 of CIN and 34 of CSCC.Results1. The percentage of HPV-16 E7 positive expression in CC, CIN and CSCC was 46.6%,70.6%and 100%, which have an increasing tendency. The differences of expression were statistically significant (P<0.01).2. The positive rate of hTERT expression in CC, CIN and CSCC was 33.3%, 58.8% and 100% respectively. The tendency was increasing. The differences of expression were statistically significant (P<0.01)3. The positive expressions of HPV-16 E7 and hTERT in CSCC were significantly related to lymph node metastasis, clinical stage and differentiation degree. The difference of expression was statistically significance (P<0.05). But the positive expression had no correlation with age (P>0.05). 4. The expression of hTERT was positively correlated to HPV-16 E7 in CSCC. (r,=0.799,P<0.01).Conclusions1.The expressions of HPV-16 E7 and hTERT were obviously increased from CC to CIN and CSCC. It indicated the important role in the development of the cervical tissues.2.The positive expressions of HPV-16 E7 and hTERT in cervical carcinoma with lymph node metastasis, high-level clinical stage and poor differentiation were significantly higher than without lymph node metastasis, high-level clinical stage and well differentiation. Given the results, we concluded that HPV-16 E7 and hTERT could play an important part in the development, progression and metastasis of cervical carcinoma.3. It was also found the expressions of HPV-16 E7 and hTERT were positively correlated, which suggested that hTERT and HPV-16 E7 might have synergistic role in the development and progression of cervical cancer.