The Biological Performance Of Ti Alloy/MWCNTs/nano-HAP Composite Material

To improve the drawback of the implant in mechanical strength,biological activity,furthermore the implant can not establish the extensive synostosis with bone tissue,theresearch put titanium alloys, multiwalled carbon nanotube and nano-hydroxyapatitetogether by the mean of microwave sintering processing in the hope of making goodcombine of favorable mechanical properties and biological activity of biologicalceramic.Through biological evaluation, to explore the early biological mechanisms ofthe combine of such material and the interface of bone, with the purpose of suppling theclinical and experimental basis for the composite material in application.Methods:1. According the relevant national and industry standards, by in vitro cytotoxicity test（GB/T16886.5-2003）,sensitization test（GB/T16886.10-2005）,hemolytic test（YY/T0127.1993）,and Oral mucosa stimulation test （YY/T0279-1995） to evaluate thebiological safety of composite materials.2. Culture the osteoblasts on the surface of composite materials for in vitro cellculture, polishing of pure titanium as the control group. Through MTT method,morphological analysis and so on to test the changes in the cell attachment,proliferationand activity expression of alkaline phosphatase when material and cells interact with eachother. 3. Implant the composite materials and polishing pure titanium implants into rabbitfemur, in the fourth and eighth weeks putting animals to death.Through the organizationevaluation to observe implant-bone interface, meanwhile understand the interactions ofimplants and bone healing.Results:1. the Ti alloy/MWCNTs/nano-HAP Composite material has good biological safety,no cytotoxicity. The results of Hemolysis test show that the material has good bloodcompatibility. The results of Mucosa stimulation test show that it do not has anystimulation for mucous membrane.Sensitization test results show that the material has nosensitization to skin.2. The Ti alloy/MWCNTs/nano-HAP composite materials can promote earlyattachment, proliferation and ALP activity of expression of osteoblast. The rough surfaceis benefit for osteoblast pseudopodia seeking attachment point to survival.3. The Ti alloy/MWCNTs/nano-HAP composite materials show good biologicalactivity and it can generate closely combination with bone tissue interface, meanwhile ithas the function of accelerating early implant stability, formation and reconstruction ofbone tissues.Conclusions:The Ti alloy/MWCNTs/nano-HAP composite materials have reliable security andgood biocompatibility,at the same time it can induce the deposit of bone-like apatite. Italso could accelerated the formation and alteration of bone tissue and shorten the time ofbone combination.