Studies On The Methylation Of GAS41Gene Promoter In Glioma

Objective:To examine the transcriptional and translational status of the glioma amplified sequence41(GAS41) in normal brain tissues and different pathological grades of glioma specimens; to investigate the methylation patterns of GAS41promoter in the development of gliomas.Methods:(1) Immunohistochemical staining was used to detect the expression of GAS41in4cases of gliomas specimens of different pathological grades.(2) SYBR Green quantitative real-time PCR was used to detect the mRNA expression of GAS41gene in3cases of normal brain tissues and6cases of glioma tissues of different grades.(3) Methylation special PCR was used to detect the methylation of GAS41promoter in3cases of normal brain tissues and12cases of glioma tissues of different grades.Results:(1) GAS41gene had a high-level of expression in gliomas tissues,(2)3cases of normal brain tissues showed significantly lower mRNA expression of GAS41compared to that of6cases of glioma specimens (P <0.05).(3) Methylation specific PCR demonstrated a higher methylation levels of GAS41in normal brain tissues compared to that in glioma tissues (P <0.05).Conclusion:(1) GAS41may play an important role in gliomas tumorigenesis.(2) Decreased methylation of GAS41promoter may be involved in gliomas tumorigenesis.