Methylation Of EDNRB Gene In Glioma

Object:Methylation of Tumor-suppressor gene promoter is one of the important epigenetics mechanisms, participating in the glioma events. This study adopts nest PCR (nPCR) and hydrogen sulfite salt after modification sequencing method (BSP method) detecting candidated tumor-suppressor genes EDNRB gene promoter methylation in surgical resection of gliomas and area this group of patients postoperative patients receied follow-up survival, discussing the abnormal EDNRB gene promoter methylation gliomas occurrence and development, the relationship between role and clinical disease material.Methods:Selected the 20 patients with glioma patients were not line, preoperative chemotherapy and radiation, collect resection of the tumor tissue confirmed by pathology of. Meanwhile collecting 2 cases of normal brain tissue were compared, the application nPCR methods and ways to test the specimens BSP EDNRB gene promoter methylation CpG island area state.The results:EDNRB gene promoter area of abnormal methylation CpG island Low level (I - level II) in gliomas EDNRB gene promoter methylation rate to 21.43% (3/14), and the high level group (â…¢- level IV) EDNRB gene promoter methylation rate is 53.85% (7/13), are statistically significant differences between (X~2 = 4.402), P = 0.037, shows that EDNRB gene promoter methylation in malignant gliomas, high degree in the more common. Age, sex, patient tumors pathological types, tumor size, and tumor location with EDNRB factors such as the genetic methylation no obvious correlation. conclusion:EDNRB gene methylation is cancer early events, and tumor progression basic business. The research did not find EDNRB gene methylation and of the patient's age, gender, cancer pathological types, tumor size factors related. .