Function Analysis Of Differential Protein On Chililling Stress Response In Cinnamomum Bodinieri Levl Leaves

Cinnamomum bodinieri Levl is the Cinnamomum species of Lauraceae. Itis not only the excellent timber species in subtropical area, but also the preciousecological environment protection and city greening tree species. Cinnamomumcamphora is thermophilic, so it is extremely difficult to introduction to the north forlow temperature. Before there has been a great deal of researches on cold resistance ofCinnamomum camphora, and have tentatively identified that Cinnamomum bodinierihas stronger cold resistance. Then these studies were mainly on physiological andbiochemical level. The study on cold resistance of Cinnamomum camphora in themolecular level has not been reported. The study in this paper explored the molecularmechanism of Cinnamomum camphora cold resistance from protein leve, it isimportant for screening cold resistance of Cinnamomum camphora.This study used proteomics techniques (two-dimensional electrophoresis, massspectrometry), the total protein of Cinnamomum bodinieri seedlings under lowtemperature stress of-14℃for2h performed a differential proteomic analysis, toexplore the mechanism of resistance to low temperature. The results are as follows:In this experiment, we analyzed the changes of differential proteins inCinnamomum bodinieri leaves under-14℃for2h. An optimized protocol wasdeveloped paying special attention to the analyses of leaf total protein onCinnamomum bodinieri based on2-DE. The optimization included samplepreparation, electrophoresis conditions etc. It is a very important platform to studyproteome of Cinnamomum bodinieri. The finally2-DE results showed that under low-temperature stress, a total of65protein spots were detected, among these proteinsports20protein spots have a good repeatability and the expression changed at leastat2-fold. These protein sports were identified by mass spectrometry. The result of19protein obtained peptide mass fingerprinting and function prediction effectively, only18protein spots could not be identified. Among them,3protein spots weredown-regulated,1were up-regulated,12protein spot expressed in control then didn’tin treat and3spots exoressed in treat then didn’t in control. There are6proteinsinvolved in glycolysis,3protein spots with unsaturated fatty acid synthesis,1proteinsand radical scavenging activity, the remaining9proteins involved in other functionsof cold resistance physiological process test. The19identified protein spots contain2unnamed protein and2hypothetical protein. The results indicated that these proteinswere involved in many metabolic processes.According to the present research situation, we can also make a further study on theprotein identified, to find the mRNA encoding the protein, then find relevant genes.Through the relevant technology transfer related gene into Cinnamomum bodinieri,observing the cold hardiness of the offspring.