| The paper mainly dealt with the study of propagation methods of Disporum bodinieri (Levl.et.Vnt.) Wang et Tang for exploiture of the wild resources. The steam of seedlings were taken as explants to establish the technique system of tissue culture. We study the technique of cutting propagation, including thc influence of exogenesis hormones treatment, the hormones' concentration, the hormones' soaking time and the seasonal difference on cuttage rooting. The results were as follows:1 The study of tissue culture :①The best way of sterilization was 0.1%HgCl2 for 6 minutes.②April is the optimum season to get explants. In this month, the germination rate is high, it is 82.35%, and the contaminated rate is the lowest, it is 71.69%.③Initial culture: initi al medium of steam: 6-BA 1.0mg/L+2,4-D0.01 mg/L+ NAA0.1 mg/L+ IBA1.0mg/L, in which the germination rate is 85.20%.④Efficiency seedling induction medium: 1/2MS+ IBA0.05mg/L, in which the germination rate is:62.26%.⑤Rooting culture:以1/2MS+NAA1.0mg/L+AC5g/L, in which the rate of rooting reached 96.13%.⑥Proliferation medium: 6-BA 0.5mg/L+KT1.5 mg/L +NAA 0.2 mg/L+ sucrose 30 g/L were the optimum medium. The multiplication rate is 6.1.⑦Forging seeding transplanting: the transplant medium in perlite,vermiculite and sands by 1:1:2 is the optimal for Disporum bodinieri (Levl.et.Vnt.) Wang et Tang ,which the ratio of survival is 82.06%.2 The study of cuttage propagation :①Different types of hormones have efficient influence on rooting, the rooting ratio is better than CK. The cuttings were treated by NAA100mg/L for 6h is the best, in which the rooting ratio is 78.46%,the length is 4.86cm,the quantity of roots is 4.76.The results of ASD and IBA were almost the same ,considering the physiology index②For NAA concentration and soaking time combination, the results show: 100 mg/L dipping in 8h,which rooting ratio is 76.9%.③The seasonal difference: the optimum season is April in spring, which the rooting rate is 75.43% in 38.3days.July in summer at the middle, October in autumn is the worst. |
PDF Full Text Request