Epidemiological Investigation Of Dairy Goat Brucellosis Of Three Counties In Yunnan Province And Kappa Analyze Of Serological Tests

Members of the genus Brucella are causative agents of brucellosis, a widespread disease ofvarious animal species, and a common zoonotic infection of man.1990s, they carry out acomprehensive brucellosis prevention and treatment monitoring in Yunnan Province, ShilinCounty is given as brucellosis re-infected area in Yunnan Province, Jianshui County is given asthe surveillance county of brucellosis. All infected persons breed are detected dairy goat bymonitoring goats, the results show that it is an important factor that dairy goats infectbrucellosis in Yunnan province. Brucellosis diagnostic tests have etiological diagnosis,serological testing and molecular biology. Brucellosis diagnostic tests have etiologicaldiagnosis, serological testing and molecular biology. Etiological diagnosis is the "goldstandard", but it has time-consuming, the potential risk of infection and prones to subjectiveawareness impact. Etiological diagnosis is not conducive to the processing and rapid diagnosisof a large number of samples. In recent years, the molecular typing study of Brucella is in-depth.Brucella species identification has stable and effective technology, and the MLVA genotypingtechnology and MLST genotyping technology are given attention and application in subtypeidentification analysis. In regard to the large-scale quarantine of the livestock brucellosis,serological tests are always important and convenient method and have widely applied in thegrass-roots level. In May2012, the State Council issued the "national long-term animal diseaseprevention plan (2012-2020)", brucellosis are prevented and treated as a priority diseases, anddifferent endemic areas of brucellosis are made the appraisal standards of prevention andtreatment. Therefore, there is a difficult question whether the" GB/T18646-2002, Diagnostictechniques for animal brucellosis " can be applied to the large-scale testing work aboutbrucellosis.1. Epidemiological investigation of dairy goats brucellosis of three counties in YunnanProvinceBy questionnaire survey, dairy goat farmers need to enhance the understanding ofbrucellosis, to improve feeding patterns and breeding status quo in Shilin County, Luxi Countyand Jianshui County, the introduction of new varieties requires timely and quarantine antiGiovanni van disease infection. They need to quarantine and prevent brucellosis for the introduction of new varieties. The actual number of serum collected is5002, test groups are89.The individual positive rate of dairy goats in brucellosis was the highest in JianshuiCounty(p=7.88%), the grop positive rate of dairy goats in brucellosis was the highest in LuxiCounty(p=55.56%), the results showed that the brucellosis infection of dairy goats was sporadicin27villages, and the individual positive rate was higher (p=6.32%). The individual positiverate and group positive rate of dairy goats in brucellosis were lowest. There were two strainsisolated from10abnormal pulses, named YN-1and YN-2, and they were Brucella melitensisbiovar3by biochemical tests, single-phase-specific serum A, M, R agglutination test andphage lysis test. The results showed that it was an important factor that dairy goats infectbrucellosis in Yunnan province, B.melitensis biovar3is the existence of livestock brucellosisinfection and the important type of occupation personnel infection in Yunnan province.2. The Kappa analyze of five serological tests of brucellosisThe antibody positive rates of five serological tests in1311serum were no significantdifference in statistics. The consistency of RBT, iELISA and FPA were very high(KRBT/iELISA=0.9395, KRBT/FPA=0.8976, KiELISA/FPA=0.9101, p<0.01), while the same resultshowed between SAT and cELISA(KSAT/cELISA=0.9071, p<0.01).3. MLVAand MLST typing of BrucellaPanel1identified that two strains of YN-1and YN-2were Brucella melitensis in MLVAgenotyping of Brucella. The clustering evolutionary analysis showed that the results wereinconsistent between traditional phenotypic identification and genotyping. Their sequence andthe number of repeating units in panel1and panel2A were consistent while their04,09,16locis’were not. MLST genotyping of Brucella showed the YN-2strains had been belong to ST8type, cobQ locis of strain YN-1as a new allele was numbered8, and the type ST of strain YN-1was numbered ST28. The results were also inconsistent between traditional phenotypicidentification and genotyping.