Study On SSR Marker And Tagging Of Male Fertility Restorer Gene Of Cytoplasmic-Nuclear Male-Sterile Line Njcmsia In Soybeans

Taking the advantage of heterosis is an effective way to improve yield for soybean. The genetic research of fertility restoration of cytoplasmic-nuclear male sterility has significant value for taking the advantage of heterosis. In recent years, with the rapid development of molecular marker technology, the use of molecular markers for gene location has become an important approach for the study of the fertility genes. Among the numerous types of molecular marker, due to the advantages of simplicity, low cost, high repeatability, good stability and the codominant advantages, SSR marker is widely used in gene location studies.Soybean cytoplasmic-nuclear male-sterile line NJCMS1A was obtained by a combination （N8855×N2899） with sterile plant F1as female parent and N2899as male parent after continuous muti-generation backcross breeding. N2899was its maintainer line of the same type. This study investigated the fertility restoring genes of soybean cytoplasmic-nuclear male-sterile line and SSR marker. The main results are as follows:The inheritance of fertility restoration of soybean cytoplasmic-nuclear male-sterile line NJCMS1A was studied using the F1, F2and F2:3of combination of NJCMS1Aand Nannong19-15. The result showed that the fertility restoration inheritance of NJCMS1A was stable in different years. F1was all fertile. F2has fertility separation, and the separation ratio of which was3:1between fertile and sterile plants. The ratio between home and separation coefficient of the fertility segregation in F23was3:1, which consisted with the result of χ2test1:2. The results indicated that the fertility restoration inheritance of NJCMS1A in the combination of NJCMS1A and Nannong19-15was controlled by a pair of dominant gene.720individual plants F2of combination NJCMS1AxNannongl9-15were selected randomly as members for SSR molecular marker.923pairs of SSR primers were used to conduct polymorphism screening on parent,332of which showed polymorphism. These332pairs of SSR primers were chose to conduct screening on the sterile gene pool （S pool） and the fertile gene pool （F pool）,14pairs of which showed polymorphism. These14pairs of primers were used to conduct screening on NJCMS1A, Nannong19-15, S pool and F pool,7pairs of which showed polymorphism. Finally, the7pairs of primers were used to amplify the NJCMS1A, Nannong19-15, S pool, F pool, Fi and F2, five of which were found to behave as codominant markers. Satt398, Satt152and Sat₂38were detected by χ2testing to perform with1:2:1separation, and the other2markers, i.e., Satt430and Sat₂16showed partial separation. The results of the linkage analysis carried out by MAPMAKER/EXP3.0software showed that Satt152and NJCMS1A had fertility restoring gene linkage. Thus, the restoring gene was located between Sat₀84and Sat₂08on the N linkage group with a distance from these two markers1.2cM and1.7cM.