Ultimately Also Five Soup Early Pregnant, Cell Respiration Suppression And Hemolysis Spectrum Toxicity Studies

Purpose:In this paper,our previous research of Buyanghuanwu decoction against cerebral ischemia neuronal injury spectrum efficiency, on the basis of the studies, the establishment of a multi-target toxicity of Buyanghuanwu decoction vitro fingerprints and Buyanghuanwu decoction spectrum toxicogenomics mathematical model. Buyanghuanwu decoction extraction and alcohol precipitation liquid as a model drug, based on the number of blood drug residue method and Hill dose-effect equation, the principle of joint mathematical and statistical analysis and spectrum toxicogenomics mathematical model has been established, seeking calculate Buyanghuanwu decoction multi-component and multi-target corresponding to the spectrum toxicogenomics.Methods:The paper based on Hill dose-effect and combined with principal component analysis, multivariate linear fitting and nonlinear curve fitting to establish Chinese medicine complex Buyanghuanwu decoction vitro associated mathematical model. HPLC fingerprint of different solubility parameter method to get Buyanghuanwu decoction polar segment active ingredient groups, and active ingredients group to establish the different polarities segment of Buyanghuanwu decoction. Homogeneous design and evaluation Buyanghuanwu decoction liquid extraction and alcohol precipitation prescription toxicity, calculated alcohol precipitation prescription toxicity extraction and alcohol precipitation liquid principal component indicators of physiological interaction coefficients, alcohol precipitation prescription toxicity the alcohol precipitation solution set of square spectrum toxicogenomics relationship.Results:1. Set up is simple, accurate and reliable RP-HPLC method for determination of liquid filling Yang five soup also mention alcohol sink the fingerprint determination method:Ultimate XB-C18column (250mm×4.6mm,5m), mobile phase:water (10%acetic acid):acetonitrile gradient elution.2. Got ultimately sank five soup also mention alcohol toxic liquid spectrum learn mathematical model, and calculate the corresponding link parameters. For example,TGF-β1to get the effect coefficient of the three main ingredients for the K1=1.69*103V1=-1.34*107, K2=-4.32*102, V2=-0.243, K3=5.42*10V3=7.1683. For BYHWD spectrum efficiency and spectrum toxicogenomics association study to calculate the similarity of0.0590.4. As for the chromatography fingerprint in vitro,ingredient8、15、10、37、42have been considered the maximum Toxic effects ingredient group in the study of Buyanghuanwu Decocation. Conclusion:This study had validated the established spectrum toxicogenomics preliminary mathematical model of Chinese herbal compound composition, which could be used in the Chinese herbal composition effectiveness research with multi-component and multi-target characteristics, and provide new ideas and experimental basis to the modernization of traditional Chinese medicine.