Stilbene In Bamboo Party Monomer Saponin And Curcumenol Content Of Mgc - 803 Gastric Cancer Cells Inhibit The Research

Objective:Gastric cancer is one of the highest fatality and malignant tumor in China, ranked2nd in the incidence of tumors. To find effective drugs and methods is the most urgent research topic on the treatment of gastric cancer. Studies show that gastric carcinogenesis is a multi-stage, multi-factor process. Its occurrence and development are not only abnormalities of proliferation and differentiation, but also related to the disorders on apoptosis. To explore the induction of apoptosis becomes an important strategy in recent years. Traditional Chinese medicine is an important part in the medical therapies of gastric cancer. It shows great significance to reduce the recurrence rate of postoperative gastric cancer, reduce the adverse effects of radiotherapy and chemotherapy, prolong survival and improve patients’quality of life. Clinical studies show that Qizhu decoction designed by Prof. Shan Zhao-wei does well in the inhibition of tumor growth and reduction of recurrence. However, the mechanism is not entirely clear yet. The objective of our study is to observe the inhibition of curcumol and dioscin and their combination from Qizhu decoction on the proliferation of human gastric cancer cell MGC-803, and to investigate the active principles and anti-tumor mechanisms of Qizhu decoction.Methods:Human gastric cancer cell MGC-803is cultured in vitro. The inhibitory rate of the drugs on MGC-803proliferation is detected by MTT assay. The morphological changes of MGC-803are observed by optical microscope and electron microscope. The effects on Caspase-9of MGC-803cells are detected by spectrophotofluorimetry. The telomerase activity is detected by RT-PCR assay.Results:I.Curcumol, dioscin and their combination has the time-and-dose-dependent inhibitional effect on the proliferation of MGC-803;2.The dugs can induce apoptosis in human gastric cancer cell MGC-803, its mechanism includes activation of Caspase-9and inhibition of telomerase activity;3.Using the combination of curcumol and dioscin have a better effect than using single of the two drugs.Conclusions:Curcumol and dioscin from the Qizhu decoction might be the active principles in inhibiting the proliferation of MGC-803.