æ † Kernel Soup Flavored Acid To Simulate Motility Insomnia Rats Plasma Monoamine Neural Transmitter In The Study

1. Research ObjectivesThrough to gavage treatment of sports insomnia rat model with Jiawei Suanzaoren Decoction,observe changes in plasma monoamine neurotransmitter content and explore the mechanism ofexercise-induced insomnia and treatment of Jiawei Suanzaoren Decoction, and then look formore intervention methods to solve exercise-induced insomnia and provide a scientific basis.2.The experimental method60female healthy wistar rats were randomly divided into6groups, that is, the control group,the model group, the spontaneous recovery group (referred to as self-health group), the diazepamgroup, Jiawei Suanzaoren Decoction low-dose group (low dose group), Jiawei SuanzaorenDecoction high-dose group (high dose group), each group of10.In addition to the control groupreceived no treatment, other groups were established simulation exercise insomnia modelthrough to five weeks strength weight loading swimming training and intraperitoneal injection ofPCPA. After modeling, pick the eyeball from model rats to take blood, add heparinanticoagulation for the await measuring; the self-health group rats irrigation stomach with saline,the low-dose group and high dose group rats were given Jiawei Suanzaoren Decoction7.1g/kgand14.2g/kg irrigation stomach; the diazepam group rats were given diazepam0.2mg/Kgirrigation stomach, a total of5days.After consecutive treatment,each group rats will beeyeballed to take blood and then measure plasma monoamine neurotransmitter levels through thehigh performance liquid chromatography-fluorescence detection.3.The experimental results3.1After the model group rates modeling by five weeks strength weight loading swimmingtraining and intraperitoneal injection of PCPA, compared with the control group, plasma5-HTand5-HIAA content decreased significantly (P <0.05); plasma NE and DA content is extremelyincreased significantly (P <0.001).3.2Compared with model group, after Jiawei Suanzaoren Decoction irrigation treatment, the ratsplasma5-HT and its metabolites5-HIAA content increased obviously (P <0.01), NE and DAcontent significantly reduced (P <0.01)3.3The weight and appetite of control group rats gradually growth on average every week. Theweight and appetite of other groups has declined after modeling, and then increased after JiaweiSuanzaoren Decoction irrigation treatment.4. Conclusion4.1The model rats plasma5-HT and its metabolites5-HIAA content decreased, NE and DAcontent increased and reduce sleep, awakening increase, the results show that the five weeksstrength weight loading swimming training and intraperitoneal injection of PCPA build athletic insomnia animal model success.4.2After JiaweiSuan zao ren Decoction treatment, athletic insomnia rats plasma5-HT and5-HIAA content increased, NE and DA content decreased, show that the JiaweiSuan zao renDecoction may be through the regulation monoamine neural transmitter content approach totreatment of exercise-induced insomnia.4.3JiaweiSuan zao ren Decoction in the treatment of athletic insomnia curative effect is distinct,the treatment effect is better than that of western medicine diazepam, and the dose-effectrelationship is obvious in a certain range.