| Objective: The rates of pregnancy with hyperthyroidism is as high as0.2‰to2‰.If the status of hyperthyroidism during pregnancy is poorlycontrolled, excessive thyroid hormone and various thyroid autoantibody couldthrough the placenta, premature delivery, palace growth retardation issignificantly higher than normal, by this way it could affect the growth anddevelopment of the fetus, meanwhile it brings about serious consequences toorgans mature of the offspring. Nestin is the fourth intermediate filaments ofcytoskeleton protein. Nestin plays an important role in the assembly andadjustment of the cytoskeleton structure. Nestin is mainly expressed incytoplasm of mature sterol cell and primary foot processes. Oxidative stress,ischemia, hypoxia and other adverse factors could reduce the content. Nuclearfactor kappa B plays an important role in regulating gene transcription startand rate. The dimer NF-κB P65-P50is the main activation form of nuclearfactor kappa B. Under normal circumstances, NF-κB P65only exists in thecytoplasmic, while it seldom is expressed in the nuclear. Ischemic anoxia,oxidative stress and other adverse factors could make the NF-κB transfer tothe nuclear. Through the admeasurement of nestin expressed in offspring’skidney and of NF-κB P65expressed in cell nucleus, to discuss the relationshipof the mother merger hyperthyroidism and progeny’s renal damage,furthermore, to provide theoretical basis for the prevention and treatment ofprogeny’s renal disease.Methods: The female SD rats were randomly divided into normal controlgroup (N group), mild hyperthyroidism group (group A) and severehyperthyroidism group (group B). The rats in A group and B group were givendifferent doses of levothyroxine sodium lavage to manufacturinghyperthyroidism model, the rats in normal control group were given the same amount of saline, continuous lavage21days. After hyperthyroidism model iscreated successfully, according to the female and male ratio2:1, and femaleSD rats are closed into the same cage with male rats. The female rats wereproceed to be filled the stomach until delivery. To observe the mothers’ andtheir offspring’s general character. Weighing offspring’s’ body weight andkidney weight in0day,21days and60days detection offspring’s thyroidfunction, kidney function in21days and60days. Observe young rats kidneypathological structure in0days,21days and60days by HE dyeing andobserve kidney ultra structure by transmission electron microscopy in21days,To measure the offspring rats’ kidney nestin and cell nucleus of NF-κB P65expression content the by the means of western blot technology in threeperiods.Results:1The mother rats’ general character and thyroid function1.1The mother rats’ general characterThe mother rats’ activity, diet quantity and water quantity in mildhyperthyroidism group was slightly increased than normal control group, thestimulation reaction to the outside more sensitive. The mother rats’ activity,diet quantity and water quantity in severe hyperthyroidism group wasincreased significantly than normal control group, the stimulation reaction tothe external environment change irritability, autism, each bite, urine increased,bowel movements are thin paste, there was high metabolic syndromeperformance.1.2The mother rats’ thyroid functionThe mother rats’ serum FT3, FT4were significantly increased in mildhyperthyroidism group and severe hyperthyroidism group than that of innormal control group, and the mother rats’ serum FT3, FT4were significantlyincreased in severe hyperthyroidism group than that of in mildhyperthyroidism group; The mother rats’ serum TSH Mild hyperthyroidismgroup and severe hyperthyroidism group were significantly lower than that ofin normal control group, and The mother rats’ serum TSH in severe hyperthyroidism group were reduced than that of in mild hyperthyroidismgroup.2The offspring rats’ general character, thyroid function and renal function2.1The offspring rats’ general characterThe offspring rats in normal control group growth of good, andgradually sprouting, the good color and luster, mild hyperthyroidism groupand severe hyperthyroidism group of young rats after birth than normal controlgroup weight loss, the growth is slow, matt, severe hyperthyroidism group ofyoung rats such performance is more apparent.2.2The offspring rats’ thyroid function2.2.1The offspring rats’ thyroid function in three groupsThe offspring rats’ content of FT3, FT4in serum in21days and in60days in mild hyperthyroidism group and severe hyperthyroidism group ishigher than that of in normal control group. The offspring rats’ content of TSHin serum in21days and in60days in mild hyperthyroidism group and severehyperthyroidism group is lower than that of in normal control group.21daysFT3, FT4severe hyperthyroidism group is mild hyperthyroidism groupincreased, TSH a mild hyperthyroidism group to reduce.60days FT3, FT4mild hyperthyroidism group than normal control group increased, TSH thannormal control group to reduce, but this was not statistically significant.60days FT3, FT4severe hyperthyroidism group than normal control groupincreased, TSH than normal control group to reduce.60days FT3, FT4severehyperthyroidism group is mild hyperthyroidism group increased, TSH a mildhyperthyroidism group to reduce.2.2.2The offspring rats’ thyroid function in three periodsNormal control group60days FT3, FT4and21days no statisticaldifference. Mild hyperthyroidism group FT3, FT460days than21daysreduced, there is severe hyperthyroidism group FT3, FT460days than21daysto reduce, no statistical significance, TSH is21days rise, no statisticalsignificance.2.3The offspring rats’ renal function In21days mild hyperthyroidism group and severe hyperthyroidismgroup of BUN and Cr was significantly higher than that of normal controlgroup.21days severe hyperthyroidism group of BUN and Cr a mildhyperthyroidism group increased significantly.60days mild hyperthyroidismgroup and severe hyperthyroidism group of BUN and Cr was significantlyhigher than that of normal control group.60days severe hyperthyroidismgroup of BUN and Cr a mild hyperthyroidism group increased significantly.Normal control group (n=12)21days with your group60days comparison,BUN and Cr no significant difference. Mild hyperthyroidism group of60daysand21days group comparison, BUN and Cr have obvious rise; Severehyperthyroidism group of60days and21days group comparison, BUN andCr have increased significantly.3The offspring rats’ body weight, kidney weight, HE dyeing and transmissionelectron microscopy3.1The offspring rats’ body weightIn0day, the offspring rats’ body weight in mild hyperthyroidism groupand severe hyperthyroidism group is lower than that of in normal controlgroup, have statistical significance. Moreover, the offspring rats’ body weightin severe hyperthyroidism group is lower than that of in mild hyperthyroidismgroup, with statistical significance. In21days, the offspring rats’ body weightin mild hyperthyroidism group and severe hyperthyroidism group is lowerthan that of in normal control group, have statistical significance. Moreover,the offspring rats’ body weight in severe hyperthyroidism group is lower thanthat of in mild hyperthyroidism group, with statistical significance. In60days,the offspring rats’ body weight in mild hyperthyroidism group and severehyperthyroidism group is lower than that of in normal control group, havestatistical significance. Moreover, the offspring rats’ body weight in severehyperthyroidism group is lower than that of in mild hyperthyroidism group,with statistical significance.3.2The offspring rats’ kidney weight in three periodsIn0day, the offspring rats’ kidney weight in mild hyperthyroidism group and severe hyperthyroidism group is lower than that of in normal controlgroup, have statistical significance. Moreover, the offspring rats’ kidneyweight in severe hyperthyroidism group is lower than that of in mildhyperthyroidism group, with statistical significance. In21days, the offspringrats’ kidney weight in mild hyperthyroidism group and severehyperthyroidism group is lower than that of in normal control group, havestatistical significance. Moreover, the offspring rats’ kidney weight in severehyperthyroidism group is lower than that of in mild hyperthyroidism group,with statistical significance. In60days, the offspring rats’ kidney weight inmild hyperthyroidism group and severe hyperthyroidism group is lower thanthat of in normal control group, have statistical significance. Moreover, theoffspring rats’ kidney weight in severe hyperthyroidism group is lower thanthat of in mild hyperthyroidism group, with statistical significance.3.3The offspring rats’ renal tissue HE dyeingIn normal group young rat0days renal tissue has not yet fully grown,renal mesangial cells and endothelial cells less, glomerular volume smaller.21days and60days renal organizational structure in order, renal tubule andglomerular in alignment, sterol cell structure normal, endothelial cells withoutedema and deformation, basement membrane without thickening. Mildhyperthyroidism group of young rat0days renal tissue has not yet fully grown,renal mesangial cells and endothelial cells less, glomerular volume smallerrenal tubule and glomerular in alignment, and normal group0days kidneytissues have no obvious difference.21days in the kidney tissues of renal tubuleand glomerular structure a little disorder, basement membrane slightlythickened, endothelial cells without edema and abnormal;60days kidneytissues a fibrous tissue hyperplasia, fibrosis than normal group obviouslyduring the same period. Endothelial cells and mesangial cell edema andvolume increase. Severe hyperthyroidism group of young rat0days renaltissue has not yet fully grown, renal mesangial cells and endothelial cells less,glomerular volume is small, and the normal group0days kidney tissues haveno obvious difference.21days renal organization arrangement anomaly disorders, there are a large number of red blood cells infiltrating, congestionedema is more apparent, basement membrane thickening obvious, matrixincrease.60days renal tissue fibrous tissue obvious hyperplasia, fibrosissignificantly. Endothelial cells and mesangial cell edema and volumeincreases a lot of inflammatory cell infiltration.3.4The offspring rats’ renal tissue by transmission electron microscopy in21daysIn normal group,21days young rat glomerular sterol cell structurearrangement, complete foot processes clear arrangement orderliness, Mildhyperthyroidism group21days young rats glomerular basement membranemild thickening, and uneven thickness, capillary hole is uneven increase,Severe hyperthyroidism group21days young rat kidney sterol cell obviousswelling, sertoli cell mitochondria sheath part or most of the fusion, part ofmembrane fusion or disappear, mesangial cells and endothelial cell swellingobvious, mesangial matrix increase, capillary window diameter increasedsignificantly.4The offspring rats’ renal tissue’ nestin expression quantityIn0day, climate mild hyperthyroidism group and severe hyperthyroidismgroup nest protein expression was significantly lower than that of normalcontrol group, severe hyperthyroidism group a mild hyperthyroidism groupnest protein expression significantly reduced.21days mild hyperthyroidismgroup and severe hyperthyroidism group of nestin than normal control groupsignificantly reduced, and the severe hyperthyroidism group nest proteinexpression is mild hyperthyroidism group significantly reduced.60days, mildhyperthyroidism group and severe hyperthyroidism group of nestin thannormal control group significantly reduced,60days severe hyperthyroidismgroup of nestin is mild hyperthyroidism group significantly reduced.Normal control group young rat0days and21days and a60days, nestprotein expression no obvious difference. Mild hyperthyroidism group21daysand60days nest protein expression is0days were significantly lower,60daysthan21days express obviously decrease. Severe hyperthyroidism group21 days and60days nest protein expression is0days were significantly lower,60days than21days express obviously decrease.5The offspring rats’ renal tissue NF-κB P65expression in the nucleusNF-κB P65expression in kidney in mild hyperthyroidism group andsevere hyperthyroidism group was significantly higher than that of normalcontrol group, severe hyperthyroidism group a mild hyperthyroidism groupwas significantly higher NF-κB P65expression. In21days mildhyperthyroidism group and severe hyperthyroidism group of NF-κB P65normal control group was significantly higher.Conclusion:1Mother suffers from hyperthyroidism during pregnancy, can cause tochildren hyperthyroidism. Hyperthyroidism severity with is related to kidneydamage. Mild hyperthyroidism will with the passage of time, will be back tonormal. As time goes on, severe hyperthyroidism, the thyroid hormone willdrop slightly, but still higher than normal.2Mother suffers from hyperthyroidism during pregnancy, can makechildren sufficient cell nest in protein expression decreased. With the degreeof hyperthyroidism aggravate, less nest protein expression. And as time goeson, the expression of nestin reduce degree will be more obvious.3Mother suffers from hyperthyroidism during pregnancy, can makechildren renal tubular interstitial cell of NF-κB nucleus transfer occurs, therenal tubular interstitial cell nuclei of NF-κB P65expression increases. Withthe degree of hyperthyroidism aggravate, less nest protein expression. And astime goes on, the expression of NF-κB P65reduce degree will be moreobvious. |
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