Expression Of Nestin In Human Glioma Tissues And Interaction Between PTEN And Nestin

Objective:To investigate the expression and significance of nestin in the slices of human gliomas. To investigate the expression of nestin in the human glioma cell lines of SWO-38 and its subseries called SWO-Z1 and SWO-Z2, and to discuss the regulation between PTEN and nestin in cell differentiation of tumor microenvironment.Methods:The average optical of nestin has been detectied in 53 human gliomas slices by immunohistochemistry with the detection by image analysis software called Image-Pro Plus 6.0, and then analyse the correlation between nestin's expression and the malignant grade. The changes of cell's appearance were observed by HE staining with light microscopy. The expression of nestin and its changes induced by si-RNA of PTEN were detected by immuno fluorescence and Western Blotting in the cell lines of SWO-38 and its subseries, SWO-Z1 and SWO-Z2.Results:1. Nestin is expressed in the tumor cells of 53 human gliomas slices, and it's roughly paralleled between the intensity of nestin immunostaining and the malignancy grade of the gliomas:more malignant, more expression. Nestin is also expressed in the vascular endothelium cells of tumor:the average opticals of groupⅠ-Ⅱgrade,Ⅲgrade andⅣgrade are 0.3292±0.1393,0.6147±0.1194和0.6361±0.1210, respectively; there are significances between groupⅠ-Ⅱand groupⅢ, or groupⅠ-Ⅱand groupⅣ,P<0.01; but no significance between group groupⅢand groupⅣbecause P>0.05.2. All of the SWO-cell lines don't have bands in 250kD where nestin should appear. They're different in the nestin expression between the SWO-cell lines because SWO-Z1 has a band of nestin in about 130kD where the other two don't have. Nestin is expressed in all the SWO-cell lines in 130kD after the treatment with si-RNA of PTEN, which has a little increase in SWO-Z1 cells.Conclusion:Nestin's expression is roughly paralleled with the malignancy grades of gliomas, no matter in tumor cells or vascular endotheliums of tumor. Nestin could be an important index which reflects malignancy and prognosis of gliomas. There is difference of nestin's expression in SWO-cells lines before and after PTEN was interfere by si-RNA, which suggests there is variance of nestin's expression in different cell differentiated state.This indicates that PTEN could influence expression of nestin. We suggest that SWO-Z1 is the lower differentiation subseries of SWO-cell lines. The SWO-cell lines offer a good model of cell differentiation for the future studies.