| Object1. Using the bone morphogenetic protein-2, cyanoacrylate and chitosan and others to made the compound biological glue. To Study the physical and biological properties of this compound. To prepare the model of a Wistar rat mandibular defect by remove part of bone of mandibular of rat.2. Putting the compound biological glue into the model of the mandibular defect of rats, to research the ability of hemostasis, adhesive and bone regeneration of the compound biological glue.Materials and methods1. The production of BMP-2with cyanoacrylate biological glue.2. The compound of temperature responsive chitosan hydrogel.3. To prepare the model of mandibular defect of Wistar rat.4.54Wistar rats were randomly divided into3groups, experimental group, control group and blank control group. The experimental group was implanted by the compound BMP-2with cyanoacrylate biological glue, while the control group was implanted with temperature responsive chitosan hydrogel, and the blank control group was implanted into nothing. The wounds were sutured layered and perfectly. To observe the ability of the hemostasis and adhesive of the compound BMP-2with cyanoacrylate biological glue during and after surgery.5. The disposal and detection of samples:The rats were anesthetized to death after the operation of implantation for3,6,9weeks. The mandibular samples of rats were examined by soft X-ray and processed by Image-Pro Plus6.0software. Routine histological observation:The joint specimens were exposed; fixed by neutral formalin, colored by HE staining, wrapped and cut into slices by4μm, study and observed them under a optical microscope.6. Statistical analysis:All the data were processed by SPSS17.0software, one-way ANOVE analysis was used to analysis. There was a statistically significant difference between experimental group and other groups.Results1. The compound BMP-2with cyanoacrylate biological glue was compounded successfully. This biological glue is semi-solid, illiquid, and elastic.2.The temperature responsive chitosan hydrogel is pale yellow, liquid and it may be become solidification glue in the37℃water for about1min.3. The mandibular defects model of rats were implanted by the compound BMP-2with cyanoacrylate biological glue, the experimental group was repaired with the wound bed and showed excellent hemostasis result.4. All the rats in the study were healthy without immunological reaction after implantation. The bone regenerated active were very obvious after operation. Soft X ray films confirmed that the defect area in the experimental group was narrower than other groups, after3,6,9weeks of implantation. No inflammation or rejection response was detected during the experimental in54rats, and the wound was healed well. The bone defect area of residual mandibular defects in the experimental group were all smaller than the other two groups after6,9weeks of operation. And the control group was smaller than the blank control group. There was a statistically significant difference between the experimental and the other two groups (P<0.05). Histology observation: There was a lot of newborn capillaries, osteoblasts, and a few lymphocytes are discovered after3weeks; Capillaries and collagenous fiber reduce, while there was a few bone tissue than the other groups after6weeks; There was a mass of bone tissue in the defect area after9weeks.ConclusionsThe compound BMP-2with biological glue has good biocompatibility, safe, stability and ability of osteoinduction. It can be used to regeneration and restruction of rat mandibular bone defect and have a good future in clinical applications. |
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