Experimental Study On Repair Of Mandibular Defects In SD Rats With Composite Scaffold Of HA/GO/BMP-2/VEGF

Objectives: Produce a porous artificial composite scaffold by using hydroxyapatite(HA),graphene oxide(GO),bone morphogenetic protein 2(BMP-2)and vascular endothelial growth factor(VEGF)as basic materials(HA/GO/ BMP-2/VEGF).Then adopt the composite scaffold to the repair of mandibular defects in SD rats with the method of tissue engineering in order to evaluate the feasibility of adopting this scaffold materials for mandibular bone defects repairing.Methods : 1.Producing the HA/GO/BMP-2/VEGF composite scaffold;2.Observing the morphology of the scaffold composite through scanning electron microscope(SEM);3.Detecting the biocompatibility of HA/GO/BMP-2/VEGF composite through MTT and Alkaline phosphatase(ALP)tests;4.Animal experiments: create mandibular defect operation areas as the same sizes as that of relative premade composite scaffolds,followed by reconstructing defects with the usage of these corresponding groups of materials(group A: HA/GO/BMP-2/ VEGF;group B: HA/GO/BMP-2;group C: HA/GO/VEGF;group D: HA/ BMP-2/VEGF;group E: control group).The experimental rats were observed after being implanted with these materials into their mandibular defects.Experimental rats were executed after 4,8 and 12 weeks respectively,and then the mandibular were observed and compared via different aspects such as general observations,X-ray tests and histological morphology.Results: The HA/GO/BMP-2/VEGF composite scaffold was safe and nontoxic.Its porous structure and the role of growth factor was favorable to the proliferation of new blood vessels and new bones.The scaffold also has good mechanical properties and biocompatibility.Conclusions: It can be concluded that the HA/GO/BMP-2/VEGF composite scaffold can be further researched and adopted as a new material for mandibular defect reconstruction.