Effects Of Human NGF Gene Transfection On Human Periodontal Ligament Cells

ObjectiveExplore the effects of human nerve growth factor genetransfection on human periodontal ligament cells. For it to become theseeds of periodontal tissue regeneration of cells lay the foundation.MethodsThis article through the liposome carrying the carrier willpcDNA3.1of nerve growth factor into the periodontal cell， through theimmunofluorescence and Western blot to prove its expression in thecells. And then the MTT, ALP by means of the analysis of thebiological characters, statistical analysis using SPSS13.0package.Result1.Through observation. Immunofluorescence NGF transfection ofthe PDLC composites in the cytoplasm is dark red, not carryingcytoplasm a red light. Transfection proof existing in the cells NGF.2. Western blot product identification carrying cell, carryingempty plasmid cells and are not carrying cell in13KD appear aspecific strip, not carrying and carrying empty plasmid cells stripnarrow color is light, carrying group article color deep bandwidth3. Two days before that, MTT cell proliferation, no significantchanges from the third day until the seventh day of the PDLCs NGFtransfection group and the other two groups of cell proliferation isobvious (p <0.05), and the other two groups have no obvious (p>0.05)changes, the difference was not statistically significant.4. ALP group content transfection group that PDLC composites compared with the control have higher trendConclusionhuman nerve growth factor gene transfection on humanperiodontal ligament cells. can promote cell alkaline phosphataseactivity enhanced， And detect the MTT cell proliferation capacityobviously improved. human nerve growth factor gene transfection onhuman periodontal ligament cells is expected to get functionstrengthened PDLC composites of seed cells, to explore gene therapyand tissue engineering in combination of periodontal tissue defect toprovide theoretical basis for the application.