Expression And Rugulation Changes Of Phospholipid Transfer Protein In Placenta In Patients With Gestational Diabetes Mellitus

Objective:To investigate the change of PLTP in the serum of GDM mothers and their neonatesand to find out the localization of the expression of phospholipid transfer protein (PLTP) inthe placenta and the variatotion of the PLTP expression in placenta.MethodsAccording to the diagnostic criteria of gestational diabetes industry amended andpromulgated in2011by China ’s Ministry of Health, selected normal pregnant women20cases, gestational diabetes mellitus patients16cases with37-41weeks pregnancy. Theconcentrations of serum PLTP in16GDM and20healthy mothers and neonates wereexamined by ELISA, and the activity levels of PLTP were assayed by PLTP Activity AssayKit used a Fluorescent enzymatic method;the expression of PLTP in placenta samples wasmeasured by immunohistoehemistry and the level of PLTP mRNA in placentas wasexamined by reverse transcription-polymerase chain reaction(RT-PCR).Results1.There was no significant differences between normal pregnancy group and GDMgroup in age, blood pressure, gestational age and pre-pregnancy body mass index (P>0.05); comparaed with normal pregnancy group,GDM group had statistically significantdifferences in fasting glucose (FGB), postprandial2hour glucose (2hPBG), fastinginsulin (FINS), insulin resistance index (HOMA-IR) and antenatal body mass index andgestational weight gain values (P<0.05);2.There was no significant differences between normal pregnancy group and GDMgroup in maternal serum PLTP content and fetal cord serum PLTP content (P>0.05),The maternal serum PLTP activity in GDM group was significantly higher than that innormal pregnancy group(P <0.05);3.Both the content and activity of PLTP in the the serum of fetus umbilical cord blood were higher than those in the maternal blood,the difference are statisticallysignificant(P<0.01);4.PLTP expression in the placenta was mainly localized in the placental vascularendothelial cells,placental PLTP gene expression levels in the GDM group wassignificantly higher than the normal pregnancy group (P <0.05);5.In GDM, fetal cholesterol, HDL-triglycerides and phospholipids were elevatedcompared with controls(P <0.05).Conclusion1.The levels of PLTP activity increased in GDM group which suggested that PLTPmay be involved in the occurrence and development of GDM;2.Placental vascular endothelial cells increase PLTP released into the fetal bloodcirculation,which to some extent,may lead to elevated levels of PLTP in the fetal bloodcirculation;3.PLTP was mainly expressed in the placental vascular endothelial cells of placenta,and placental PLTP mRNA expression increased in the GDM group;4.High PLTP activity in fetal serum may enhance conversion of HDL intocholesterol-accepting particles, thereby increasing maternal-fetal cholesterol transfer.