Researches On Extraction And Purification Process And Primarily Pharmacological Activities Of Total Saponins From Nodding

ObjectiveTo research extraction and purification process of total saponins fromnodding euphorbia; Observation in the best purification process of the totalsaponins anti-inflammatory and antioxidant effects.MethodsTake the single factor experiment and the orthogonal experiment methodsto determine the total saponins of nodding euphorbia for the best extractioncondition.with the total saponins were complete index; Take the macroporousadsorption methods to resin and solvent precipitation of total saponins, and themain factors affecting purification; The rat balloon synovial inflammation modelwere used for determination of the indicators to study the total saponinsnodding euphorbia anti-inflammatory and antioxidant effects.ResultsThe best extraction process for ethanol concentration is80%, and theextraction temperature of70℃, than for the1:8material liquidand theextraction time is1.5hours for the total saponins from Nodding euphorbia;total saponins of Nodding euphorbia purification effects for AB-8type is thebest of macroporous adsorption resin type, purification of nodding euphorbiatotal saponins product purity is63.2%; After purification of the total saponins from nodding euphorbia had shown some of the anti-inflammatory effects, butno significant antioxidant effects.ConclusionsTake the ethanol extracts methods for the total saponins of noddingeuphorbia is feasible, after solution precipitation and macroporous adsorptionresin method the total saponins of nodding euphorbiacan effectivelypurification, of purified total saponins have certain anti-inflammatory, but noantioxidant effect performance at present.