The Effect Of Leptin, Ghrelin With Obstractive Sleep Apnea Hypopnea Symptom

Objective This paper will explore Leptin and Ghrelin in patients with obstructive Sleep Apnea Hypopnea syndrome (Obstractive Sleep Apnea Hypopnea Symptom OSAHS) influence. First through the observation, high-fat diets to rat blood Leptin and Ghrelin concentration changes the influence of the distribution and its receptor expression, clarify both between obesity in rats in the process of forming role; Then through the observation in intermittent hypoxia state obesity rats in Leptin and Ghrelin blood of change, reveals both in the role of OSAHS process. Obstructie sleep apnea hypopnea syndrome is a systemic disease, is the common basis of pathology many diseases, in recent years, its incidence upwards, harmfulness, all existing treatments are not definite effect, so from the mechanism research OSAHS of occurrence, development, for clinical diagnosis and treatment OSAHS provide new acting targets have great significance.Methods Buy58male youth health SD rats about160g±10g, adaptability to feed a week later randomly divided into two groups, namely the normal control group (foundation feed, n=28) and obesity induction group (high fat feed+ordinary feed mixing feed, n=30), weekly record weight, length, then the control group and the high fat rats, according to whether low oxygen each divided into two groups, often oxygen group each group only12, low oxygen group of each group only16, according to the requirements given low oxygen intervention. In the forth and eighth weekend random take six rats, were enrol your biometrics, lung and blood fat tissue from YongMei league immune method to detect plasma Leptin and Ghrelin content, with RT-PCR, the Western-Blot methods detection Leptin and Ghrelin receptors, protein in adipose tissue, lung tissue of expression differences.Results ELISA results show that the plasma Leptin and Ghrelin concentration in in obesity, low oxygen and time three factors under the influence of the different levels of performance, eight group differences are significant, simple obesity, serum Leptin leels, Ghrelin levels dropped (4weeks P<0.05,8weeks P<0.01); Intermittent hypoxia and serum Leptin content reduce weekend, Ghrelin secretion increased (P<0.05); Intermittent hypoxia8weekend, Leptin level rise further, plasma Ghrelin levels increased significantly (P<0.05). RT-PCR, the Western-Blot results is different, Leptin receptor proteins in adipose tissue and the expression level and plasma concentration change present consistent, lung tissue meaningless; And Ghrelin receptors in the lungs and protein and fat tissue was not detected in meaningful results.Conclusion Plasma Leptin and Ghrelin in the expression level and obesity and low oxygen closely related to the intervention, and then show that its and obesity, obstructive sleep apnea hypopnea syndrome happen and the development of the relationship between the degree; Leptin receptor proteins in adipose tissue and the expression further confirmed the results; And was not detected result indicates that the factor of the relevant organization is too little or no expression expression, can tip the research orientation for the future.