Han Chuan-ning Oral Liquid On Experimental Asthma In Guinea Pigs With Bronchial Alveolar Lavage Fluid In Experimental Study On No And TNF-a

Objective:This paper aims at the use of Han chuan-Ning oral liquid on asthmaguinea pig model,through the observation of the asthmatic guinea pig asthmasymptoms and bronchial alveolar lavage fluid in NO and TNF-a contenteffect,evaluation of Han chuan-Ning oral liquid on guinea pig asthmainflammation curative effect, as Han chuan-Ning oral liquid and providetheoretical basis for clinical application.Methods:Experimental selection of50male guinea pigs in the same conditionadaptability of feeding for1weeks, and then randomly, each group of10, atotal of5groups, respectively is the asthma model group, normal control group,dexamethasone group, low dose group of traditional Chinese medicine andtraditional Chinese medicine in high dose group. Normal control group with1ml saline intraperitoneal injection, the remaining four groups of guinea pigswere given by intraperitoneal injection of10%saline solution1ml ovalbuminsensitized.Since the injection for fourteenth days, the asthma mode lgroup,Chinese medicine group of small dose,Chinese medicine in high dosegroup,dexamethasone group, every day, on the afternoon of the first of theguinea pig in4litres of spray cylinder, with1%albumin solution ultrasonicatomization10-20s, Excited to asthma, appears short of breath hurried, nod,prostrate, body trembling phenomenon,prompting modeling success.In guineapigs with asthma indusud by intragastric administrationsynchronization,medicine group of small dose,Chinese medicine in high dosegroup,dexamethasone group In the two shot interval time intragastricadministration，3ml each time,2times a day.Normal controls2times daily withnebulized normal saline instead of ovalbumin excitation.The normal control group and the asthma model group2times daily with3ml saline lavage, groupswere gavage for2weeks，And observation of guinea pig mental activity, dietaryintake, fur color, The size of and death。The last one hours after dosing,with1%albumin solution inhaled15-20seconds，Observation of guinea pigwheezing symptoms such as latency (accurate to s),Normal control groupreceived saline instead of.All groups except the above factors applied drugtreatment, the daily feeding method are same, be free to consume conventionalguinea pig feed and water.By detecting the guinea pig bronchial alveolarlavage fluid in NO and TNF-a level changes observed in Han chuan-Ning oralliquid treatment effect.Results:Asthma guinea pigs BALF NO, TNF-a Content than the normal controlgroup was significantly elevated, and in the Han chuan-Ning oral liquid intreatment of post, BALF NO, TNF alpha in the asthma model group wassignificantly lower than in guinea pigsConclusion: Han chuan-Ning oral liquid can shorten asthma attack, and poorblood circulation, duration of asthma，reduce the bronchoalveolar lavagefluid (BALF) of NO and TNF-α content, but its role with the drug dose related,further illustrate the Han chuan-Ning oral liquid possesses anti-inflammatory,anti-allergy, is the treatment of childhood bronchial asthma effectiveprescription, worthy of clinical application.