Study On The Uygur Medicine Compound Of The Anticancer Activity Parts Of Abnormal Black Munziq-Based On The "TCM Components" Mode

Objective: The purpose of this paper lay in the preparation of the anticancer activityparts of Abnormal Black Munziq,studied on its chemical components and thedetermined of the main components’ content,to evaluate its material composition,preparedits standard components and proceed chemical characterization,analysised the quantitativeand qualitative,created Information database. Method:1. Gradient elution on AbnormalBlack Munziq to obtain its anticancer active parts used polyamide columnchromatography and solid phase extraction technology. By using polyamide columnchromatography to prepared the extract of Abnormal Black Munziq. Used solid phaseextraction,separated the extract to prepare the anticancer activity parts.2. Prepared thestandard components of anticancer activity parts by used semi preparative highperformance liquid chromatography.3. Proceed chemical characterization with standardcomponents,used UV and HPLC.4. Analyzed the qualitative of anticancer activity parts ofAbnormal Black Munziq,used TLC.5. Analyzed on the quantitative of the anticanceractivity parts,used HPLC.6. Drew up Prescription database software and input all kinds ofinformation of Abnormal Black Munziq,established Data-base,used C#databaseprogramming language. Results:1. Acquired the anticancer activity parts of AbnormalBlack Munziq.2. Prepared18standard components of the anticancer activity parts ofAbnormal Black Munziq.3. Directed at standard components to proceed chemicalcharacterization of UV and HPLC.4. The qualitative analysis result of TLC indicatedthat the anticancer activity parts of Abnormal Black Munziq contained gallic acid、caffeic acid、rutin、licorice glycoside、quercetin-3-O-glucoside、isoferulic acid、quercitrin、hesperidin、rosmarinic acid、glycyrrhizic acid.5. The result of quantitativeanalysis indicated that every gram of the Anticancer activity parts of Abnormal BlackMunziq contained gallic acid4.85mg、caffeic acid6.99mg、rutin27.23mg、licorice glycoside20.24mg、quercetin-3-O-glucoside12.25mg、isoferulic acid16.08mg、quercitrin7.75mg、hesperidin58.16mg、rosmarinic acid57.96mg、glycyrrhizic acid19.29mg.6. Established of the information database of Abnormal Black Munziq.Conclusion:1. Abnormal Black Munziq with gradient elution,use polyamide columnchromatography and SPE is a simple and effective way to acquire its anticancer activityparts.2. To a complex things,the most simple and direct way to understand isdecomposition and simplification. Semi preparative high performance liquidchromatography,which has the feature of high-efficiency,speediness and automaticoperation,has an important position in the area of separation and purification.3.Systematic chemical characterization of Traditional Chinese Medicine composition,is away of using the modern separation technology and method,to proceed scientific andeffective characterization research. UV and HPLC,which play an important role in the areaof complex samples analysis,are also the most powerful tools to solve the problem of thearea.4. The anticancer activity parts have high content of flavonoids substances.Qualitative analysis by TLC,have advantages of shortcut,trace,high sensitivity and goodseparation efficiency and so on.5. On the anticancer activity parts of Abnormal BlackMunziq undertook quantitative analysis of gallic acid、caffeic acid、rutin、licoriceglycoside、quercetin-3-O-glucoside、isoferulic acid、quercitrin、hesperidin、rosmarinicacid、glycyrrhizic acid,use HPLC. The method’s accuracy,precision and so on are allconsistent witht the requirement of content determination,is a special and practicalquantitative analysis method.6. Use the information database to analysis and characterizethe Chinese herbal medicine and compounds of traditional Chinese medicine,supplyevidences for quality control of medicine and standard components,provid informationsupporting platform for the high-efficiency and in-depth research of traditional ChineseMedicine’s pharmacodynamic material basis and the development of Components oftraditional Chinese Medicine.