Expression Of Nestin In Human Fetal Tissues In Different Stages

Objective:To observe the morphology changes of lung, kidney, esophagus, stomach, small intestine and large intestine during the fetal development, to detect the expression and changes of nestin in lung, kidney, esophagus, stomach, small intestine, large intestine tissue and thus speculate the function of nestin during the fetal development.Methods:Collect the tissues of lung, kidney, esophagus, stomach, small intestine, and large intestine from human fetuses of3-8months. All the samples were made into paraffin sections and then divided to two groups. In one group, HE staining was used to find out the change of morphology in development. For another group, we performed the immunohisto chemistry to investigate the expression of nestin in the process. Therefore, we figured out the expression of nestin in development based on the semi quantitative analysis of the immunohistochemistry results.Results:Nestin was expressed in various tissues,including lung, kidney, esophagus, stomach, small intestine, and large intestine during fetal development. It was expressed in the bronchial side, pulmonary interstitial and blood capillary while it is negative in the epithelium of pulmonary conductive portion. Nestin was expressed in renal corpuscle and blood capillary but not in renal tubule. Meanwhile, nestin was also expressed in the blood capillaries of lamina propria, submucosa and muscularis layers in digestive tract.The expression of nestin in different tissues was decreased with the development of fetus.Conclusion:Nestin was expressed in various tissues in human fetal development. And the expression level were changed with the development of fetus which indicated the relationship of nestin with the fetal development. Nestin was expressed in capillary of different tissues; however, the expression levels were decreased with the fetal development which indicated that nestin might play a role in angiogenesis.