Cofilin Signaling Pathways In Osteoarthritis Effects On Mechanism Of Research

Purpose: This topic research contents of basic research， the purpose is made by theanimals in vitro culture， little cartilage cells of continued to mechanical stress effect， toconstruct mechanical force induced osteoarthritis occurred early cartilage cells model， labfor molecular and protein level detection， reveals cofilin signaling pathways in cartilagecells， the role of stress transmission， observation cofilin signaling pathways inosteoarthritis of the regulation effects of cartilage cells of cartilage cells and the influence ofthe skeleton restructuring. For Cofilin signaling pathways in the role of diseaseosteoarthritis theoretical foundation and to provide the basis.Method: Through the separation of cartilage cells of rabbit and the original generationsto cultivate， through the MTT method validation cell growth cycle， application MTTmethod to determine the cell growth curve， to verify the stick wall， a blue and collagentype II aniline immunohistochemical stains in cell appraisal，according to the training of thecartilage cells for phenotypic appraisal result， cartilage cells that is more active， keep theoriginal cell activity， is the ideal cells.The static pressure method to construct mechanical force of success inducedosteoarthritis occurred early cell model of morphological observation. Model and furtherthrough the morphology， and a blue staining aniline， Ⅱ collagen type andimmunohistochemical method operation flow cytometry test parameters such as cell cycle andapoptosis proof model building success.Under aseptic conditions， take the experimental group and control group， littlecartilage cells in cell total RNA extraction， parallel and PCR amplification transcriptase areusing RT-PCR and Western blot test Cofilin in osteoarthritis cartilage cells of gene expressionand protein expression， sure Cofilin signal pathway to participation in osteoarthritis damagepathogenesis.Result: This experiment through the RT-PCR and Western blot test Cofilin gene andprotein expression， in mechanical force Cofilin induced osteoarthritis occurred earlycartilage cells model group cells are high and expression， the control group compared withnormal cartilage cells， significant difference （P <0.05）. Tip cofilin signaling pathways involved in the pathogenesis of osteoarthritis， but the specific mechanism is still needsfurther research.