Cofilin Signaling Pathway In The Absence Of Fracture And Dislocation Of Cervical Spinal Cord Injury Research

Purpose: Through this project after the animal model, laboratory testing of molecular and protein levels, Cofilin pathway to further reveal the fracture and dislocation in the absence of disease in the cervical spinal cord injury the role of the theoretical basis and provide experimental basis.Methods: By repeat the manufacture of animal models, from the choice of two goats in the experimental group, under sterile conditions and 846 under general anesthesia, using nickel-titanium screw screwed into the front of the C5 vertebral body diameter of 3.5 mm titanium screws, in the fifth week After the successful production of a sheep model of chronic spinal cord compression, and from two randomly selected, in the C-arm fluoroscopy X-ray machine to determine C5, 6 intervertebral foramen and needle puncture with a shunt into the intervertebral foramen, pull out the needle core, self-made balloon pressure slowly to the pressure syringe injection of contrast agent, thus causing acute posterior compression. And the behavioral assessment, and CT and MRI examination were performed in the spinal cord occupancy rate and pressure within the segment of spinal cord compression situation. Under sterile conditions, taking the experimental group and control group with the sheep spinal cord segments, the cell total RNA extraction, reverse transcription and PCR amplification in parallel, respectively, using RT-PCR and Western blot detection of Cofilin in the neck without fracture or dislocation spinal cord injury gene expression and protein expression, to determine whether the signaling pathways involved in Cofilin no fracture or dislocation in the cervical spinal cord injury.Results: Application of six goats, sheep, repeated simulations, the establishment of cervical fracture and dislocation-free animal models of spinal cord injury, chronic spinal cord compression group successfully 3, the success rate of 75%, the more obvious clinical symptoms appear, the experimental group a success, a success rate of 50% appears obvious clinical symptoms of hindlimb paralysis, with the control group significantly different (P0.05). RT-PCR analysis showed no fracture or dislocation in the sheep cervical spinal cord injury, the experimental group was significantly higher than Cofilin gene expression; Western blot test results showed no fracture or dislocation in the sheep cervical spinal cord injury in the experimental group was significantly higher Cofilin expression in the control group, 157.7% for the control group, were statistically significant.Conclusion: 1. The experiment was repeated simulation of the sheep without fracture and dislocation of cervical spinal cord injury model, the experimental group showed no fracture and dislocation of the sheep and human cervical spinal cord injury changes in clinical symptoms similar to, Tarlov motor function score improved methods, X ray, CT and MRI and other testing to determine repeated simulations sheep without fracture and dislocation of cervical spinal cord injury model success. 2. Cofilin pathway to participate without fracture and dislocation in the cervical spinal cord injury.