Research Of Quality Control About Abnormal Savda Munziq Granular And α-Glucosidase Inhibitors

Objective: The qualitative and quantitative study of main active ingredient inAbnormal Savda Munziq Granular and the research of α-glucosidase inhibition, ItProvides a stable and reliable method for the quality control of Abnormal Savda MunziqGranular,providing corresponding research data for Abnormal Savda Munziq Granular’sclinical application and development Method: Using thin-layer chromatography (TLC)screening and qualitativethe main active ingredient of the Abnormal Savda MunziqGranular; Using the high performance thin layer chromatography (HPTLC) method for thedetermination of the content of rutin, rosemary acid, glycyrrhizic acid, Liquiritin; Usinghigh performance liquid chromatography method (HPLC)for the determination of rutin,using High Resolution Rapid Resolution Liquid chromatography method RRLCdetermines the content of rosmarinic acid, glycyrrhizic acid, liquiritin; using the part of theextract of the four Abnormal Savda Munziq Granular,which is Petroleum ether, ethylacetate, ethanol, water has a test on α-glucosidase inhibitory activity and examined byBio-luminescence photometer Results: using ultrasonic extraction, determines Rosmarinicacid and rutin in Abnormal Savda Munziq Granular qualitatively and quantitatively withethyl acetate-formic acid-acetic acid-water (15:1:1:1.5) as developing solvent; ethylacetateacetate-formic acid-acetic acid-water(15:1:1:1.7) as the developing solventdetermines glycyrrhizic acid and liquiritin in Abnormal Savda Munziq Granularqualitatively and quantitatively, The test sample with the standard, or control medicine’schromatography spots have the same color and Rfvalues, Negative control has nosignificant interference,there are no large differences about rosmarinic acid, rutin, licoriceglycoside, glycyrrhizic acid’squantitative results with Liquid chromatography; water partof the extract of Abnormal black Munziq has a good α-glucosidase inhibitory activity,Petroleum ether extract part has no inhibitory activity. Conclusion:The topic using TLC,HPTLC, HPLC, RRLC determines Rosmarinic acid, rutin, licorice glycoside, glycyrrhizicacid in Abnormal black Munziq qualitatively and quantitatively, using bio-luminescence photometer determines four extraction sites of Abnormal black Munziq ‘s inhibition forΑ-glucosidase enzyme activity, The method specificity, simple, accurate, can be used forthe Abnormal black Munziq’s qualitative and quantitative identification, also provides areliable method for quality control of the abnormal black Munziq...