| Nitric oxide (NO) is a new cell-messenger molecule with strongbiologic activity and has become the latest hot spot in the field oftransfusion medicine. It is a gas molecule with free radical nature andcombined with Hbβ93Cys to form SNO-Hb, which could go through theerythrocyte membrane. NO bounded with Hb can regulate NO storageand release to mediate vasodilation. The main effective factors oferythrocyte deformability are cell volume, cell shape, cytoplasmicviscosity and membrane mechanical properties. Although the lipid bilayerand the cytoskeleton of RBC can affect the mechanical behavior oferythrocyte membrane, the physical properties of membrane skeleton isthe determined factor of the membrane viscosity and elasticity. Adversechanges of banked blood have been extensively studied and confirmed. Inparticular, the decline of RBC deformability leads to the dysfunction ofstored blood, this result of change may jeopardize the safety of clinicalblood transfusion and may be even harmful for the potential recipients.Therefore, how to improve the hemorheology of banked blood hasbecome imperative traits matter. The interaction of NO and hemoglobinmay lead a series changes, which is from the conformation change of RBC membrane skeleton protein to the change of RBC deformability.This study is trying to improve the deformability of RBCs by adding NOand to explore the effect characteristics of NO on RBC deformability.Objective: To explor mutual relations of NO and erythrocytedeformability by studying the effective characteristics of RBCdeformability with different NO concentrations added to NO donor inbanked blood during different times of storage. Methods: Bloodsamples (200mL/bag) were collected from the ten healthy men.20mLblood from each samples were distributed to five tubes (4mL/tube). Fourtubes of blood samples were incubated with four different concentrationsSNP separately in8-phase (3h,1d,3d,5d,7d,14d,21d,28d) of storagetimes. SNP concentrations were1μM (A group),5μM (B group),10μM(C group),50μM (D group), separately. The last tube was the control onewithout any treatment. At the same time,1mL blood sample drawn fromblood bag was used to detect the NO concentration at each time, Themultiparameter analysis of hemorheology (high-shear viscosity, low shearviscosity, Casson viscosity, hematocrit, erythrocyte deformability index,aggregation index, stiffness index) was been tested for each group.Results: Hematocrit of control blood samples had no significant changesin storage period. Erythrocyte deformability index, aggregation index andstiffness index were gradually increased with time prolonged duringstorage of banked blood. Whole blood relative viscosity in high-shear was significantly increased at1d, and then maintained the trend ofincreasing. Whole blood relative viscosity in low-shear was no changefrom3h to5d, and significantly increased at7d, and then maintained thetrend of increasing. Casson viscosity was significantly increased at1d,and then also maintained the trend of increasing. When the SNPconcentration was5μM or10μM，erythrocyte deformability index in7dor more than7d storage of banked blood cells was significantly degraded.Higher than the best range of NO concentrations, the erythrocytedeformability index was significantly increased. When the SNPconcentration was5μM, erythrocyte rigidity index was changedsignificantly compare to the control group from3d to7d. When the SNPconcentration was10μM, erythrocyte rigidity index was changedsignificantly compare to the control group from14d to28d. When theSNP concentration was50μM, erythrocyte rigidity index was changedsignificantly compare to the control group after5d. The erythrocyteaggregation index with a control value did not significantly change whenblood was incubated with different SNP concentrations. Conclusion:Hemorheology traits of banked blood was changed significantly duringstorage of banked blood. Erythrocyte deformability index andaggregation index were decreased significantly with time prolonged. NOcould significantly impact on the erythrocyte deformability withdual-phase. When NO was at an optimum concentration, erythrocyte deformability was improved significantly. Higher than the bestconcentration, erythrocyte deformability was damaged. The storagequality of banked blood could be improved significantly by adding thebest concentration of NO. |
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