| Objective:To observe the effects of sevoflurane and propofol on High-shear of whole blood viscosity, Middle-shear of whole blood viscosity, Low-shear of whole blood viscosity, erythrocyte rigidity index(ERI), erythrocyte aggregation index(EAI) and erythrocyte defor-mation index(EDI) in the young men and then to approach the discussion of sevoflurane and propofol on the effects of hemorheology mechanism. The rusult of this study may be could to provide reference for clinical anesthesia applications.Methods:1.Subjects and group:Forty young male patients who were scheduled to be operated on epigast in QiLu Hospital of Shandong University and The Second Hospital of Shandong University with ASA grade I or II were randomly divided into two groups:sevoflurane group(Group S, n=20)and propofol group(Group P, n=20). There were similar in the basic situation in the two groups.2.Medicine, apparatus and methods:All patients were given 0.5mg atropine IM and monitored by electrocardiogram, arterial blood pressure, pulse oxygen saturation(SpO2), nasopharyngeal temperature(T) and end-tidal carbon dioxide partial pressure (PETCO2). Anestheia was induced by sevoflurane,2μg/kg fentanyl, 1-2mg/kg cisatracurium (Group S) or 2 mg/kg propofol(Group P) and maintained with 2-3μg·kg-1·h-1 fentanyl, cisatracurium, 1%-3% sevoflurane (Group S) or 4-6μg·kg-1·h-1 propofol(Group P). Intraoperative blood was not transfused and there was no use of diuretic drugs. Venous blood was taken suction at pre- anesthesia(T1) and 60 min after post-operated (T2).3ml venous blood was used to measure High-shear of whole blood viscosity (200s-1 shear rate), Middle-shear of whole blood viscosity(30s-1 shear rate), Low-shear of whole blood viscosity(5s-1 shear rate), ERI, EAI and EDI in Department of Inspection, The Second Hospital of Shandong University, lml venous blood was added to 4ml 0.9% normal saline and cold-storageed in -80℃refrigerator. The enzyme activity was measured by Kit Manual(the established companie, Nanjing, China).3.The design is completely random design, and t test was used if homogeneity of variance while t1 was used if variance uneven for the measurement of data by SPSS 15.0 analysis. P-values less than 0.05 were considered to represent statistically significant differences.Result:1.There were no differences in HR, MVP, SpO2, T and PETCO2 between the two groups before operation (p>0.05). There were no differences in HR, MVP, SpO2, T and PETCO2 at T2 than those at Tl in the two groups (p>0.05)2.There were no significantly different effects observed on the High-shear of whole blood viscosity, Middle-shear of whole blood viscosity Low-shear of whole blood viscosity(p>0.05). The ERI, EAI and EDI were lower at T2 than those at T1 in Group S (p<0.05). The activity of Ca2+Mg2+ATPase increased from(5.49±0.97)μmol Pi·107RBC-1·hour-1 to(5.57±1.00)μmol Pi·107RBC-1·hour-1 (p>0.05) while the activity of Na+K+ATPase decreased from(5.50±1.57)μmol Pi·10RBC-1·hour-1 to(5.32±2.02)μmol Pi·RBC-1·hour-1 (p>0.05) in Group S from T1 to T2.3. No signficintly different effects were observed on the Low-shear of whole-blood viscisity in Group P (p>0.05). The ERI, EAI and EDI were higher at T2 than those at Tl in Group P (p<0.05). The High-shear of whole-blood viscisity and Middle-shear of whole blood viscosity were lower at T2 than those at T1 (p<0.05) in Group P.The activity of Ca2+Mg2+ATPase decreased from (5.51±0.96)μmol Pi·107RBC-1·hour-1 to (4.97±1.06)μmol Pi·107RBC-1·hour-1 (p>0.05) while the activity of Na+K+ATPase decreased from (5.53±1.78)μmol Pi·107RBC-1·hour-1 to (4.19±1.66)μmol Pi·107RBC-1·hour-1 (p<0.05) in Group P from T1 to T2.Conclusion:There is no influence on the whole-blood viscisity by sevoflurane but it can increase the EDI in the young men while propofol decreases the EDI and the High-shear of whole-blood viscisity and Middle-shear of whole-blood viscisity. |
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