The Effects Of MicroRNA-194Mimics On Proliferation、Migration And Invasion Of Breast Cancer Cells In Vitro

Objective：In the background of MCF-7and MBA-MD-231breast cancer cell lines,we usegene transfer techniques and functional experiments to investigate the effects ofmiR-194mimics on proliferation、migration and invasion of breast cancer cells.Wetry to explore biological function of miR-194in breast cancercells preliminarily。Methods:The miR-194mimics was transfected into MCF-7and MDA-MB-231breast cancer cell lines by lipofectamine2000,and then the expressions ofmiR-194were elevated.MTT and soft agar assay were used to detectcellular proliferation.Wound healing and transwell assay were used todetect cellular migration and invasion.Results:MTT assay showed that MCF-7and MDA-MB-231breast cancer cells withoverexpression of miR-194are more likely to have poor proliferation in contrast tocontrol groups（P<0.01）,and this phenomenon was more obviously in MCF-7.Soft agar assay revealed that MCF-7with overexpression of miR-194had lowerplating efficiency than the control cells（P<0.01）.Wound healing and transwell assay revealed that the migration and invasion ofMDA-MB-231in experiment group were obviously inhibited in contrast to thecontrol group（P<0.01）. Conclusions:Making miR-194were overexpressed in MCF-7and MDA-MB-231through genetransfer techniques,and then observed that cellular proliferation、migration andinvasion were obviously inhibited.The miR-194mimics played an important role ascancer-supressor gene in vitro.