The Role Of MiRNA-429 On The Biological Behaviors Of Breast Cancer Bcap37 Cells And The Related Research

Background and purpose Breast cancer is a heterogeneous disease, ranking second in the female cancer-related mortality, including a large variety of different clinical features. In the past decade, a large number of studies have confirmed the molecular subtypes of breast cancer: luminal A subtype, luminal B subtype, HER2 over-expression subtype, basal-like subtype, normal cell-like subtype or other molecular subtypes. Luminal subtype is related with ER + and / or PR +; HER2 over-expression subtype is related with over-expressing HER2 receptor and 17q12-21 gene amplification; Basal-like subtype is defined by the basal / myo-epithelial marker expression and related with ER-, PR- and HER2-. Importantly, the molecular subtypes have different specific m RNA expression profiles, and these specific m RNA expression profiles due to the expression of different mi RNAs. Compared with breast cancer tissue and adjacent tissue, mi RNA disorders are common patterns in breast cancer, suggesting the importance of mi RNA disorders in the development of breast cancer. Mi R-429 belongs to a mi RNA family that includes mi R-200 c, mi R-141, mi R-200 b and mi R-200 a. Mi R-429 plays a normal biological function in vivo, specifically expressed in human embryonic stem cells. The abnormal expression of mi R-429 affects the function of tumor stem cells and a variety of tumors. In this study, we found that the expression of mi R-429 is in a low level in human breast cancer cell lines Bcap37, and further studies showed over-expression of mi R-429 promotes human breast cancer cell Bcap37 proliferation and migration, thus contributing to the development and metastasis of breast cancer.Methods 1. Real-time PCR was used to detect the expression level of mi R-429 in human breast cancer cell line BT20, BT474, Bcap37, SK-BR-3, MCF7, T-47 D and MDA-MB-231; to select a cell line with lowest expression level of mi R-429 as our object of study. 2. Hsa-mi R-429 overexpression lentivirus infected human breast cancer cells Bcap37 to establish stably transfected cells. 3. Real-time PCR was used to detect the expression level of mi R-429 in both the experimental group and control group cells to determine the transfection efficiency. 4. MTT assay, scratch test, Transwell experiments was used to observe how mi R-429 affacted Bcap37 proliferation, migration, metastasis, etc. 5. Real-time PCR was used to observe how mi R-429 affacted the transcription level of FBXW11, GLI3, RAP1 B, CSNK1G3, MAP3K5, MAP4K3, PPM1 B and other gene-related m RNA.Results The expression of mi R-429 was significantly increased after breast cancer cell line Bcap37 was infected with hsa-mi R-429 overexpression lentivirus(P <0.01). The cell proliferative capacity of Bcap37 cells was enhanced after overexpression of mi R-429. The cell migration capacity of Bcap37 cells was increased after overexpression of mi R-429 as demonstrated by cell scratch test and transwell experiment, the difference was statistically significant(P <0.01).Conclusion Overexpressed mi R-429 can significantly increase cell proliferation and migration in human breast cancer Bcap37 cells.