Prevention And Cure Function Of Balsalazide And Roles TLR2and TLR4on The Experimental Terminal Ileitis

Objectives:1. Proposed side-to-side intestinal anatomists in the ileum-caecum to establish theexperimental terminal ileitis animal model observe the prevention and cure function ofthe balsalazide and study the expression of TLR2and TLR4in ETI with or witnoutbalsalazide.Methods:One hundred clean level and healthy male SD rats were randomly divided intofive groups, the normal contral group (A), the model group (B), balsalazide50mg.kg-1.d-1group (C), balsalazide75mg.kg-1.d-1group (D) and balsalazide100mg.kg-1.d-1group (E). each group had20SD rats. Group B to group E were becarried operations such as ileum-caecum side-to-side intestinal anatomists, while thenormal control group were be anesthetized. After operated two weeks，Group C, Dand E respectively received balsalazide levitation liquid50mg.kg-1、75mg.kg-1、100mg.kg-1each1ml irrigation stomach. Group A and B received saline1mlirrigation stomach processing everyday as contral. Observe the general state after theoperation. At postoperative4,8weeks, these animals were be killed respectively, getthe terminal ileum of these rats, take HE and IHC stains, observe all groups of thepathological and histological change, and then count the inflammation scores in nakedeyes and through microscopiclens and intestinal intraepithelial lymphocyte. Toobserve the positive expression of TLR2and TLR4.Results:1. The general state of every groupAt postoperative3-5days, all the opertion SD rats were weaker than before, andthe weight descends obviously, there have statistically differences between the modelgroup, balsalazide groups and the control group (P<0.05)righe now. All the SD ratsweight recovered at postoperative from2to4weeks.4weeks later. There were no statistically differences in mortality (P>0.05).2. Ileum naked eye inflammation scoresThere were acute inflammation give priority to performance in all operation SDrats at4weeks, but model group was more serious than balsalazide groups (P<0.05).Along with the increase of balsalazide therapeutic doses, C-E groups inflammationdegree reduce in turn, score were statistically differences (P<0.05). At8weeks, thescores of model group were increased to maximum. Yet balsalazide groupsinflammation degree reduce in turn, B-E groups score were statistically differences(P<0.05).3. Organizational microscopically inflammation scoresThere were acute inflammation give priority to performance in all opetion SDrats at4weeks; the HS score in group B was significantly highter from groups A,C,Dand E (P<0.05). Along with the increase of balsalazide therapeutic doses, the HS scoreof groups C to E inflammation degree reduce in turn(P<0.05).At8weeks, chronicinflammatory performance in group B, C,D and E.the HS score in group B wassignificantly highter from other groups (P<0.05); Yet in group C, D and E theinflammation gradually reduce. Compared C,D and E groups there were significantdifference (P<0.05).4. Count intestinal intraepithelia lymphocyte in terminal ileumAt4weeks, the count intestinal intraepithelia lymphocyte in group B was higherthan other groups(P<0.05). Groups C-E higher scores than group A(P<0.05); Alongwith the increase of balsalazide therapeutic doses, the count intestinal intraepithelialymphocyte of groups C to E was reduced in turn (P<0.05).At8weeks, Group Blymphocyte managed the inflammatory cells in the through microscopic lens.compared with A and C-E groups there were significant differences (P<0.05). groupsC to E count intestinal intraepithelia lymphocyte reduced in turn(P<0.05). the lightestinflammation in group E,Compared with A, the were no significant differences(P>0.05).5. TLR2and TLR4expression in terminal ileum At4weeks, model group TLR2and TLR4positive expression was higher thangroups A,C,D and E (P<0.05); Groups C-E TLR2and TLR4positive expression werehigher than group A(P<0.05), Along with the increase of balsalazide therapeutic doses,groups C to E TLR2and TLR4positive expression were reduced in turn(P<0.05). At8weeks, compared with groups A and C-E, group B TLR2and TLR4positiveexpression was increased（P<0.05）; Groups C-D TLR2and TLR4positive expressionwere higher than group A(P<0.05); Yet group E TLR2and TLR4positive expressionwas close group A (P>0.05).There was positive relationship between TLR2and TLR4positive expression and microscopically inflammation score(P<0.05).Conclusions:1. Balsalazide can prevention and cure function on the experimental terminalileitis,and dose-response relationship in time.2. The positive expressions of TLR2and TLR4were increased in the process ofthe experimental terminal, and Balsalazide on experimental ileum treatment functionof probably with its block or inhibit bowel tissue TLR2and TLR4signaltransmission and the immune response.