Effect Of S100A8/A9Protein Complexes On Proliferation、 Invasion And Metastasis Of Human Cervical Cancer Caski Cells

ObjectiveInvestigation of S100A8/A9on human cervical carcinoma CasKi cell proliferation andinvasion and metastasis of influence, to study the inhibited proliferation and invasiveability of action on S100A8/A9on CasKi cells.MethodsIn vitro culture of human cervical carcinoma CasKi cell line,10μ g/ml,20μg/mland30μg/ml three different concentrations of S100A8/A9and PBS respectively handlethe fourth generation CasKi cells, using a tetrazolium(MTT) assay and Transwellmigration test and comparison to proliferation and migration capacity changes of beforeand after the treatment of CasKi cells, and make a statistical analysis, select Transwellinvasion experimental concentration group. The selected concentrations of S100A8/A9and PBS respectively handle the fourth generation of CasKi cells, using Transwellinvasive assay comparison to before and after invasion ability changes of the treatmentof CasKi cell.Results⑴Results of MTT experimental show that: In comparison to the PBS controlgroup and10μg/ml,20μg/ml,30μg/ml group, CasKi cell proliferative capacitydecreased significantly (P<0.01); The48hour group,20μg/ml inhibition rate is thebiggest, and comparison to10μg/ml and30μg/ml group, CasKi cell proliferationcapacity decreased (P<0.01).⑵Results of migration experimental show that:Comparison to the control group of PBS and10μg/ml,20μg/ml,30μg/ml concentrationof S100A8/A9group, the migration of CasKi cells decreased (P<0.01). Eachexperimental group comparison,20μg/ml and30μg/ml concentration of S100A8/A9 group showed no significant difference (P>0.01), comparison10μg/ml and20μg/ml or10μg/ml and30μg/ml, The migration of CasKi cells significantly decreased (P<0.01),under the condition of the same effect, we choose a lower concentration to reduce itstoxicity, choice the20μg/ml concentration group as the invasion assay concentration.⑶Results of invasion experimental show that: Compared with the control group, CasKicell invasion ability decreased significantly (P<0.01).Conclusions⑴S100A8/A9play a role of proliferation inhibition on CasKi cervical cancer cell,the strongest inhibition is at the concentration of20μg/ml and reaction time of48hourgroup.⑵S100A8/A9can reduce migration ability of cervical cancer CasKi cell, andthe effect with the increase of concentration and enhancement.⑶S100A8/A9canweaken invasion and metastasis ability of CasKi cervical cancer cells.