Conventional Chemotherapy Drugs Combined Hyperthermic Chemotherapy The Impact Of In GDF15and TFF3Expression On SW480

Objective: Probe conventional chemotherapy drugs combined43℃hyperthermic chemotherapy in GDF15and TFF3expression on SW480.and related mechanisms.Methods：In the first place,the sw480cell strain are divided into fourgroups. Control group: Cancer cells have not been any treatment;Thermotherapy group: place culture flask in43℃constant temperaturewater bath for30minutes; The chemotherapy group: Add tegafur(concentration6μg/mL) and oxaliplatin (concentration8μg/mL) in theculture medium of SW480; Thermo-chemotherapy group: Add tegafur(concentration6μg/mL) and oxaliplatin (concentration8μg/mL) in theculture medium of SW480, and then place culture flask in43℃constanttemperature water bath for30minutes. After the four groups of cells aredisposed, Place the four groups of cells in a very stable incubator and culturfor24hours.(1) flow cytometry assay of apoptosis in SW480cells,(2)Immunohistochemical method and Western Blot detect GDF15and TFF3 protein expression;(3)Rt-PCR semi-quantitative detect GDF15and TFF3mRNA expression.Results: After conventional chemotherapy，combine43℃hyperthermic chemotherapy，SW480cells increased apoptosis, GDF15and TFF3protein levels decreased, GDF15and TFF3mRNA expression isalso reduced.Conclusion: conventional chemotherapy drugs combined hyperthermicchemotherapy enhanced proliferation and inhibition of metastasis andrecurrence SW480role by inhibiting the GDF15and TFF3protein andrelated gene expression of SW480colorectal cancer;Hyperthermiacombined with chemotherapy has a synergistic effect for inhibition ofGDF15and TFF3expression.