Apoptosis Of Liver Cancer Cells Induced By Hyperthermic Chemotherapy And Its Influence On Patients With Advanced Hepatic Carcinoma

Objestive â‘ To investigate the effect of hyperthermic chemotherapy on apoptosis and its mechanism of liver cancer cells BEL-7402. â‘¡ To investigate the effects of5-fluorourail and mitomycin c combined with 42℃ whole-body hyperthermia for advanced hepatic carcinoma.Methods â‘ Human liver cancer cell lines BEL-7402 were cultured in vitro andunderwent hyperthermic chemotherapy in 42℃. The cell growth and apoptosis were observed,the cell cycle were studied by flow cytometry,and the expression level of apoptosis regulator gene were examined by immunohistochemical method.Resultsâ‘¡16 patients with advanced hepatic carcinoma enrolled in the trial group,5-Fluorourailand mitomycin c were infused during the treatment of whole-body hyperthermia. 26 patients in control group received chemotherapy only.Results â‘ The effect of hyperthermic chemotherapy on liver cancer cells BEL-7402 was remarkable.The G₁ phase arrest and the upregulated of bax and Fas were observed after hyperthermic chemotherapy.â‘¡ The response rate (WHO standard) were 31.25% and 3.85% respectively.Conclusions â‘ The apoptosis rate induced by hyperthermic chemotherapy is higher than that of chemotherapy.The cell cycle arrest and expression of apoptosis regulatior gene may contribute to the higher level of apoptosis. â‘¡ This clinical trialdemonstrates the feasibility of performing whole-body hyperthermia combined with chemotherapy.