Relationship Between DNA Ploidy, CD133Protein Expression And The Short-Term Effects Of Radiation Therapy In Nasopharyngeal Carcinoma

Objective:To detect the expression of DNA ploidy and CD133protein in nasopharyngeal carcinoma, and to explore their relations with the short-term effects of radiation therapy.Methods:The expression of DNA ploidy and CD133protein in samples from56untreated NPC patients was detected by flow cytometry and immunohistochemistry respectively. In addition,10samples from nasopharyngeal benign lesions were also detected as control. The patients were given common radiotherapy, and the short-term effects were evaluated according to the RECIST criteria when the radiation therapy ended up. Relationship between DNA ploidy, CD133expression and the short-term effects of radiation therapy in nasopharyngeal carcinoma was analyzed retrospectively.Results:1.The positive rates of DNA aneuploid and CD133protein expression were significantly higher in NPC than in nasopharyngeal benign lesions (P<0.05).2.DNA aneuploid was related with the clinical stage and and T stage of NPC(P<0.05), but no significant correlations were found with age, gender and lymphatic metastasis(P>0.05). Expression of CD133protein was related with the lymph node metastasis of NPC(P<0.05), but no significant correlations were found with age, gender clinical stage and T stage(P>0.05).3.The radiosensitivity of DNA aneuploid group was significantly higher than that of DNA diploid group(P<0.05). And the radiosensitivity was significantly lower in CD133protein positive expression group than in CD133protein negative expression group(P<0.05).4.The short term response rate was lower in DNA aneuploid group than in DNA diploid group, but no significant correlation was found between them(P>0.05).And the short term response rate was significantly lower in CD133protein positive expression group than in CD133protein negative expression group (P<0.05).5.In56cases of nasopharyngeal carcinoma, the expression of DNA aneuploid and CD133protein was significantly positively correlated(P<0.05).Conclusion:1.The positive rates of DNA aneuploid and CD133protein expression were significantly higher in nasopharyngeal carcinoma than in benign nasopharyngeal diseases, indicating that they are probably involved in the tumorigenesis of NPC.2.Expressions of DNA aneuploid and CD133protein were closely related to the radiosensitivity of NPC, and CD133protein was also related to the short-term effects of radiation therapy, so the detection of them in NPC tumor tissues may be helpful to predict the radiosensitivity of NPC patients and to plan individual therapy.3.Expression of DNA ploidy and CD133protein in NPC was positively correlated, indicating that they are closely related in affecting nasopharyngeal carcinoma, but the relevant molecular mechanism requires further investigation.