Investigation And Chemical Composition Analysis Of Polygonati Rhizoma Resources In Different Areas

ObjectiveMastering the resource status of Polygonati Rhizoma initially, finding out the situation on exploitation, utilization and protection and determining the main distribution region and reserves. To determine the polysaccharide content in Polygonati Rhizoma from different areas and to study the effects on polysaccharide content by different drying methods. To establish the high performance liquid chromatographic (HPLC) fingerprint of Polygonati Rhizoma, in order to provide theoretical basis for the identification of different varieties and quality.Methods(1) Selecting distribution area according to the place recording in Flora of China, Chinese Materia Medica and the Flora of each place. Studing out the collection routes by referring specimens and research literature. Collecting and making Polygonati Rhizoma specimens, record the collecting location, habitat, altitude and et al. of sample.(2) The polysaccharide content was determined by the anthrone-sulfuric acid method and analyzing polysaccharide content on different varieties, different drying methods and different areas. (3) Study the Polygonati Rhizoma from different areas by utilizing the HPLC fingerprints.Results(1) Resource investigation①Polygonatum sibiricum Red. was distributed in Northeast, Northwest, North China and other provinces. Relatively, there was a high yield in Hebei Province. We find the abundance of Polygonatum sibiricum Red. resource is not as high as previously estimated in Wuchuan, Inner Mongolia Autonomous Region where there is less annual rainfall, land desertification and low vegetation coverage, so, the Polygonati Rhizoma resource tends to wear out in Hebei province and Inner Mongolia Autonomous Region.The rhizoma’s diameter of Polygonatum cyrtonema Hua was able up to5~7CM, this variety distributed in south Anhui, Yandang Shan Mountains, Pan’an in Zhejiang Province, most areas of Hunan and around the Dabie Mountains, but there was more resource distributing in Jiuhua Mountains, Jinzhai of Anhui and so on. Owing to unauthorized mining phenomenon became increasingly serious, the resource became fewer and fewer.Polygonatum kingianum Coll. et Hemsl. mainly distributed in Guizhou Province and Shizong, Chuxiong, Dali of Yunnan Province, It is difficult to find it in the wild. ②The cultivated area of Polygonati Rhizoma is too less, it was a certain scale of planting in Lueyang, Shanxi Province and Polygonatum sibiricum Red. was the main cultivars.(2) Determination for the content of solomonseal polysaccharidesThe maximum content of solomonseal polysaccharides was Polygonatum sibiricum Red. and then was Polygonatum cyrtonema Hua and the last was Polygonatum kingianum Coll. et Hemsl. Freeze-drying method was significantly better than oven-dry and the content of solomonseal polysaccharides were basically identical when the drying temperature of oven-dry meathod was50~80℃.(3) The HPLC fingerprints of Polygonati RhizomaEstablished the HPLC fingerprints of Polygonati Rhizoma from different areas, Chromatography conditions as follows:an ODS C18column (250mmx4.6mm,5μm) was employed, the gradient elution of acetonitrile and water was used as mobile phase with a flow rate of1mL·min-1, the detection wavelength was set at203nm and the column temperature was set at30℃, Detecting fingerprints of10batches of the samples. The results showed that there were13common peaks contained in the tested samples.Conclusion(1) Polygonati Rhizoma wildly distributed in China, however, Polygonatum sibiricum Red. has strong regionality, centralized distribution and all the different varieties of Polygonati Rhizoma can weakly adapt to the environment.(2) Obtaining the data of polysaccharide content in Polygonati Rhizoma. Freeze-drying method was better than oven-dry, due to the limitations of price, dry carrying capacity and other factors for the freeze-drying equipment, this method can not been widely used in the actual processing and production of Polygonati Rhizom; Although dried with different temperatures, the solomonseal polysaccharides was extremely stable.(3) The HPLC fingerprints of diosgenin in Polygonati Rhizoma show good repeatability, and the method is not only simple but easy operation, it can provide a reference standard method for quality control of Polygonati Rhizoma.