| Objective:The purpose of this study animal experiments is to investigate the exact effect of the separate application of ultrasound on knee osteoarthritis, and to explore the mechanism of ultrasound on KOA and KOA in the pathological process, and provide a theoretical basis and clinical guidance for the physical factor in the treatment of KOA reduce pain in patients with KOA, Improve there activities of daily living, and alleviate the burden of these diseases on the familyandsocial.Methods:We randomly selected40healthy male New Zealand white rabbits2.5to3kg,6to8months of age, by the random number table, randomized, controlled balanced principle is divided into blank group of16and made the module24, making the module24animals underwent right hind knee plaster cast straight brake modeling. Six weeks after the lifting of cast immobilization, and the control group and model group rabbit were randomly selected8(6weeks group (K1group) were named for the control and cast immobilization for6weeks group (group O)) the results of detection of the line model (including the right knee passive range of motion, the naked eye and optical microscope to observe the pathological changes of rabbit knee joint cartilage and score, and serum and joint lavage fluid of SOD, MDA, and MMP-3). Determine the success of the modeling, the remaining model group16animals made film were randomly divided into control group (M group), ultrasonic treatment group (C group)(n=8). Group C daily with ultrasound on the right knee treatment, the M group and the remaining eight blank group rabbit (named blank control routine feeding of10weeks (K2)), not any disposition of the4th week after treatment ends all rabbits with material related indicators measured, and for statistical analysis.Results:Statistically, the passive range of motion, visual observation of articular cartilage appearance, light microscopy of articular cartilage and its mean Mankin ’s score, joint lavage fluid of SOD, MDA and MMP-3and changes in indicators the control group6weeks (Kl group) with cast immobilization for6weeks group (O group) the difference between statistical significance (P<0.05), confirmed the success of knee osteoarthritis animal model; Six weeks of the model control group (M group) and cast immobilization group (O group) and ultrasonic treatment group (C) difference was statistically significant (P<0.05), if after the lifting of plaster fixed to any treatment, articular cartilage will further worsen the degeneration of the ultrasound treatment to slow or control the further deterioration of articular cartilage to a certain extent; the serum SOD, MDA, MMP-3content of the difference not statistically significant (P>0.05). Conclusion:Rabbit brake KOA model to better simulate the KOA in the pathogenesis of knee extension. Osteoarthritis rabbit model of knee joint fluid reduce the concentration of SOD, MDA, and MMP-3concentration. The ultrasonic treatment can delay further deterioration of the KOA model of rabbit articular cartilage degeneration. Ultrasonic treatment can increase the concentration of SOD in the KOA model of rabbit knee joint fluid, reducing the concentration of MDA and MMP-3, blocking the development of KOA, KOA have clinics. Ultrasound for the treatment of KOA to play to relieve symptoms and improve the role of the disease. |
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