Expression Of ING1and Mutant P53in Human Brain Gliomas And Its Significance

Background and ObjectivesGlioma is a common primary intracranial tumors of the nervous system, with surgical recurrence rate, high mortality rate and poor prognosis feature. Surgery remains the main treatment, postoperative radiotherapy and chemotherapy has been widely used in clinical, however, the treatment effect is still not ideal, the treatment of glioma is still to overcome the problem of the field of neurosurgery. To find effective is the hot topics of neurosurgery. With the development of the Molecular Biology of cancer, people gradually came to recognize that a number of factors involved in tumorigenesis and development, including multiple genes and many cytokine involvement, such as loss of control normal cell cycle, tumor angiogenesis, activation of oncogenes and tumor suppressor gene mutation and inactivation of tumor plays a vital role in the development process. Therefore, for genetic research may provide a new basis for the gene therapy of glioma.Garkavtsev and other have used modified cDNA the subtrahend cloning out a new gene, which is Inhibitor of growth1(ING1), it involved in promoting cell cycle arrest、cellular senescence and apoptosis、 involved in stress response、regulating DNA damage response and inhibit tumor cell metastasis、invasion and tumor angiogenesis、 the regulation of DNA repair and genome stability etc, which is a significant tumor growth inhibitory factor. Nuclear phosphorylates protein p53gene mutation or the interaction between the cancer protein and thus lead to functional loss of live, its often caused by abnormal of cell growth and promote tumor, the existing data suggest that the p53gene mutations in human tumorigenesis is the most common genetic level change. From the research on the molecular biological level, the role of ING1in glioma reported less in the country, in order to understand the ING1and mutant p53expression in human glioma and its significance. In the experiment, with the RT-PCR technique to detect ING1and mutant p53mRNA expression in human glioma, which is to explore the role of both the occurrence and development of glioma and to provide a basis for gene therapy of glioma.General materials and methodsTake from forty two cases of human glioma specimens from ZhengZhou University, the Second and the First Affiliated Hospital of the tumor tissue removed by the neurosurgeon surgery in September2010to August2011,twenty-two cases were male and eighteen female patients, aged7to75years, average age of43.5±16.4years, forty-two cases of tumor specimens are the primary disease patients, and before surgery without radiotherapy and chemotherapy, immediately after the remove of the specimens into the-80℃refrigerator to save. Diagnosis of all specimens by the Department of Pathology conventional hematoxylin-eosin(HE)staining. According to WTO classification criteria of the central nervous system tumors in2007:Grade I Ⅱ twenty-eight cases (low-grade malignant glioma),including sixteen cases of astrocytoma, subependymal ependymal tumor two cases, ependymoma three cases, and oligodendrocytes of astrocytoma four cases, oligodendrocyte cell tumor three cases. Grade III-IV fourteen cases (highly malignant glioma),including anaplastic astrocytoma four cases, anaplastic oligodendrocytes glioblastoma five cases, glioblastoma three cases, medulloblastoma two cases. Non-tumor brain tissue eight cases were sent to the control group, which were taken from patients of brain trauma, cerebral hemorrhage required decompression. The expression of ING1mRNA and mutant p53mRNA are detected in42cases human glioma tissue and ten cases non-tumor brain tissues by RT-PCR. the data were statistically analyzed by using SPSS17.0software. Inspection level is a=0.05. RT-PCR results1. Expression mRNA of ING1in human glioma The expression of ING1mRNA was1.19±0.27、0.64±0.16、0.77±0.29、0.34±0.11in the non-tumor brain tissues, glioma tissue, grade I～II and Grade III-Ⅳ.Its statistical analysis using statistical software, expression mRNA of ING1gene between non-tumor brain tissue and gliomaN I～II group and III～IV group were statistically significant(P<0.05).2. Expression mRNA of mutant p53in human glioma The expression of mutant p53mRNA was0.29±0.16、0.75±0.34、0.45±0.14、0.95±0.21,in the non-tumor brain tissues, glioma tissue, grade Ⅰ～Ⅱ and Grade Ⅲ～Ⅳ.Its statistical analysis using statistical software, expression mRNA of mutant p53mRNA gene between non-tumor brain tissue and glioma、Ⅰ～Ⅱ group and Ⅲ～Ⅳ group were statistically significant(P<0.05).3. ING1and mutant p53correlation analysis Expression mRNA of ING1gene is higher in non-tumor brain tissue, expression mRNA of p53gene is lower in non-tumor brain tissue, expression mRNA of ING1was in a high level and expression mRNA of mutant p53tended to decrease in the low-grade glioma samples, expression mRNA of ING1was in a low level and expression mRNA of mutant p53tended to increase in the high-grade glioma samples. By statistical analysis, both showing negative correlation, Pearson correlation r=0.417,(P<0.05),the different was significant.Conclusion1. Compared with non-tumor, the expression of ING1in human brain glioma was significantly reduced, the higher malignant degree of glioma, the lower expression of ING1. Compared with non-tumor, the expression of mutant p53in human brain glioma was significantly enhance, the higher malignant degree of glioma, the higher expression of mutant p53.2. Negative correlation was show between the expression of ING1and mutant p53in glioma. Reduced expression of ING1and the enhanced expression of mutant p53is not isolated incident, which is expected to provide new ideas for diagnosis and treatment of glioma.