| Gastric cancer is one of malignant tumors that have a higher morbidity and mortality rate, and it is the third highest in death from malignancies. Henan province is one of the high incidence areas of the upper digestive tract cancer. Gastric cancer is a multi-factor, multi-step and multi-stage process, it is due to the synergetic effect of genetic factors, biologic factors and environmental factors. In1995, Correa proposed that the excitation, persistent and development of inflammation played important roles in the formation of gastric cancer, and these factors involved in excitation or regulating of inflammatory may promote the occurrence or have protective effects for gastric cancer. IL-8, a member of the chemokine superfamily, is chiefly produce by neutrophils cells, and it is a neutral grain of chemokine and activating factor and it can induce angiogenesis, promoting cell motility and mitosis, and it has a great relationship with the tumor’s occur and growth, tumor invasion and metastasis.etc.Currently, there are some studies concerning about IL-8gene polymorphisms and the risk of gastric cancer, but there is a big controversy over these findings.Therefore, the aim of this study is to explore deeply the relationship between the IL-8gene and gastric cancer, and then,we carried out the study including two parts.ObjectiveTo explore the relationship of two SNP sites in IL-8gene, gene-environment interaction and gastric cancer, to provide a theoretical basis for revealing the etiology of gastric cancer and developing intervention measures of gastric cancer.MethodThe data of IL-8gene251polymorphism and risk of gastric cancer related case-control study at home and abroad from1979.1to2011.10was analyzed by Meta analysis method, and the consolidated OR value (95%CI) were conclusion.A total of228patients with Gastric Cancer and288healthy individuals were enrolled and divided into patient group and control group in this study. The polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP) was applied to detect the base transition in the IL-8-251and IL-8+781sites. SPSS12.0software was applied to analysis frequencies of genotypes and alleles. Haploview software was applied to analysis frequencie of the haplotype of those two sites.Result1. Meta analysis resultsOf14case-control studies selected for IL-8-251Meta analysis, a total of3817gastric cancer cases and5396controls were included. Compared with the wild-type homozygote TT, the pooled Odds Ratio (with95%CI) of IL-8-251genotype for gastric cancer risk AT, A A, and (AT+AA) was1.22(1.02-1.46),1.30(0.99-1.71),1.18(0.99-1.40), respectively.2. Results of case-control studyIL-8-251AA genotype can increase55%(OR=1.55,,95%CI=1.00,2.43) of the risk of Gastric Cancer than IL-8-251TT genotype. With the adjustment of smoking history and family history of cancer, interaction coefficients gamma values of IL-8-251site and IL-8+781site and drinking history were1.13and0.67, respectively. It suggested that the-251A or+781T alleles can increase or decrease the risk of drinking, and the interaction model were multiplicative model or multiplication model, respectively.Conclusion1. It was showed in Meta analysis that IL-8-251locus of the heterozygous genotype AT may increase the risk of gastric cancer, and the OR (95%CI) was1.22(1.02-1.46);2. It was showed in Case-control study that IL-8-251AA genotype can increase the risk of gastric cancer in the Han population from Henan province, the OR(95%CI) was1.55(1.00-2.43);-251A or+781T allele can increase or reduce the risk of drinking, respectively, and these two interactions be named super-multiplicative model or sub-multiplicative model. |
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