The Experments Study Of Tin Smelting Productive Dust Damage To Rats’lung Tissue

Objective:Understand the situation of tin smelting workers’pneumoconiosis, and analyzing the relationship between the time length of taking dust and pneumoconiosis disease incidence. Study the tin smelting productive dust damage lung tissue early and its degree, conjecture the possibility of the dust cause workers take pneumoconiosis disease.Methods:(1) Using cross-sectional survey research method, collect physical examination material ofall on-the-job workers in the tin smelting workshop of one smelter in Guangxi in2010and all diagnosis information of the pneumoconiosis disease patients. Using current life table method to calculate pneumoconiosis disease accumulated morbidity and workers seniority accumulated pneumoconiosis disease incidence, using SPSS16.0statistical software to analysis the relationship between the time length of taking dust and pneumoconiosis disease incidence. (2) Collet the productive dust with natural breath of settlement in the tin smelting workshop of one Guangxi smelter factor, screening respirable dust with particle size<5μm, and determine the dispersion and free silica content, makes up to50mg/ml concentration by physiological saline to take the experiment study. Choose SPF rats, random into the tin smelting dust of crude refining workshop group,the tin smelting dust of refining workshop group, the negative control group and the positive of standard quartz dust group, with1ml/single by the method of without exposure an endotracheal tube type take the dust in rats, observate pathological slices of lung tissue after30days and90days, and measured the rats’weight and the wet weight of lung,calculate the lung viscera coefficient, applicate SPSS16.0statistical software to analysis the difference of the lungs visceral index.Results:(1) the general situation and the situation of tin smelting workers’ pneumoconiosis:There are385smelting workers tin smelting workshop, men are354, women are31, and at the age of19～56years old; The total length of service in the0.17～38.00years, average total length of service16.51years; touching dust length of service0.17～36.00years,the average is15.62years There are9people who were diagnosed as pneumoconiosis disease of385tin smelting work, all were men, prealence was2.34%, patients diagnosed as age at the time of pneumoconiosis disease in34～55(39.0±5.1)years old, then dust length of service9-18(15.6±3.1)years.(2) The cumulative incidence of patient s’length service:the tin smelting workers happened pneumoconiosis disease on the time lenth of touching dust in9to18years, incidence increase gradually raised with the length of service dust, then incidence increased rapidly between13to18years of the length of service dust.(3) The of pneumoconiosis disease and the related analysis between accumulated prevalence ratewith the length of service dust:pneumoconiosis disease sick in the seniority of touching dust on the number9-23years, then it is highly relevant between length of service dust and pneumoconiosis disease accumulated rate [correlation coefficient (r)=0.933,P<0.05], regression equation is y=0.003x-0.012.(4) The free silica content of tin smelting workshop productive dust:the free silica content of crude refining workshop’s dust is53.19%, refining plant is44.25%, percentage of two workshop dust particle size<5μ m were above90%after screening.(5) The comparison of lung visceral index:After30days, it were positive difference in statistical significance (p<0.05) between crude refining workshop group with negative control group and positive control group, it were positive difference in statistical significance (p<0.05) between refining plant group with negative group, and weren’t positive difference (p>0.05) between refining plant group with positive control group. After90days, it were positive difference in statistical significance (p<0.05) between crude refining workshop group with negative group, and weren’t positive difference (p>0.05) between crude refining workshop group with positive control group, it were positive difference in statistical significance (p<0.05) between refining plant group with negative control group and positive control group.(6)The pathology observation of lung tissue:Macroscopic observation, after30days, the surface of crude refining group can see visible and scattered plaque, which center is furvous color and gray plaques around; refining plant group can see diablo and gray plaques around, irregular shape; after90days, some lung tissue of crude refining group can see gray plaques with tough texture, refining plant group can see diablo and gray plaques with tough texture.Microscope Observation, two groups are visible in the dust particles composure and focal dust formation in many bronchioles, terminal bronchi and the alveolar around the pipes, focal dust can be seen in macrophages, and have fiber mother cells, neutrophils and lymphocyte responses. After90days, crude refining group can see formation of inflammatory granuloma.Conclusion:(1) There is close relationship between length of service dust and pneumoconiosis disease accumulated rate of tin smelting workers, with the existing work conditions in the factory, if you want to put the pneumoconiosis disease control in the prevalence of about1%, it is advisable that the workers take the length of service dust with no more than7years.(2) The change of the lung tissue after30days and90days in the productive dust dust of tin crude refining tried workshop and refining plant are dust grain composure and focal dust formation, focal dust can be seen in macrophages, lymphocyte proliferation, did not see the fibrosis lesions, thick dust group also can see mild inflammation. Because of the relatively short time of observation, we still need to observe longer or do further research.